摘要
生物信息学分析表明,稻瘟病菌丝氨酸蛋白酶MoSp1在水稻中的互作蛋白OsZfp1,为含有环指结构域的C3HC4型锌指蛋白。对稻瘟病菌侵染过程中OsZFP1基因的表达动态分析表明,OsZFP1基因表达水平在稻瘟病菌Guy11孢子悬浮液接种水稻后缓慢升高,接种后18h达到最高峰,约为3.8倍,这表明该基因响应稻瘟病菌的侵染。利用改进的农杆菌介导的转基因技术成功获得OsZFP1基因的过表达植株。抗性分析表明OsZFP1过表达植株的整体抗稻瘟病能力得到显著提高。说明OsZFP1基因在水稻抵抗稻瘟病菌侵染过程中扮演着十分重要的角色。
The rice protein OsZfp1 which interacts with MoSp1 is a C3HC4-type RING-finger protein predicted by bioinformatic tools.The transcriptional changes of OsZFP1 gene during Magnaporthe oryzae infection showed that the expression level of OsZFP1 peaked with 3.8folds at 18 hours after spore-spaying inoculation,revealing that OsZFP1 might respond to M.oryzaeinvasion.The OsZFP1 overexpression transgenic rice plants were gained through modified Agrobacterium-mediated transformation.The statistic analysis indicated that the overexpression plants confer resistance against M.oryzae.In conclusion,the OsZFP1 gene may play an important role in rice immunity against M.oryzae.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2015年第2期135-140,共6页
Chinese Journal of Rice Science
基金
高等学校博士学科点专项科研基金资助项目(20113515110002)
国家科技支撑计划资助项目(2012BAD19B03)
关键词
稻瘟病菌
锌指蛋白
转基因水稻
Magnaporthe oryzae
zinc finger protein
transgenic rice.
