摘要
为了探讨核苷酸结合寡聚化结构域1（nucleotide-binding oligomerization domain containing 1,NOD1）在抗沙门氏菌感染过程中的作用,本试验采用实时荧光定量PCR的方法检测了血液中NOD1基因在转录水平的表达量变化。试验分为3组,鸡白痢沙门氏菌组、肠炎沙门氏菌组和对照组,分别在感染后1、3、5、7d检测NOD1mRNA的表达水平。结果显示,感染后的1~7d,鸡白痢沙门氏菌和肠炎沙门氏菌感染后的表达量变化趋势不同,其中,鸡白痢沙门氏菌感染后表达量呈先上升再下降然后再上升的波浪形变化,而肠炎沙门氏菌感染后的表达量呈逐渐上升趋势。与对照组相比,血液中NOD1mRNA的水平,在鸡白痢沙门氏菌感染后的3和7d的表达量显著高于对照组（P〈0.05）,在肠炎沙门氏菌感染后的5、7d的表达量显著高于对照组（P〈0.05）。提示,鸡白痢沙门氏菌和肠炎沙门氏菌感染后均可促进鸡血液中NOD1基因的表达,NOD1基因可能参与了机体抗沙门氏菌感染过程。
In order to investigate the role of nucleotide-binding oligomerization domain containing1（NOD1）during the process of the Salmonellainfection,the expression of NOD1 gene in blood was detected in transcriptional level using Real-time PCR method.Chickens were divided into three groups：Salmonella Pullorum group,Salmonella Enteritidis group and control group.The levels of NOD1 expression were detected 1,3,5,7dduring Salmonella challenge.The results showed that there were different changes of NOD1 expression from 1to 7dafter two kinds Salmonellachallenge.Wavy change was observed in Salmonella Pullorum group,gradually increase trend was found in Salmonella Enteritidis group.Compared with the control group,the expression levels of NOD1 mRNA in blood were significantly higher（P0.05）at 3and 7dafter Salmonella Pullorum infection and significantly higher（P0.05）at 5and 7dafter Salmonella Enteritidis infection.The results suggested that two kinds of Salmonellacould promote NOD1 gene expression in blood,and NOD1 gene might be involved in the body against the Salmonellainfection.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第3期531-536,共6页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金青年科学基金(31101795
31101833)
现代农业肉鸡产业技术体系岗位专家(CARS-42-GO3)
现代农业技术领域863计划(2011AA100301)
