摘要
目的:检测加味四逆散对肝纤维化大鼠肝组织的病理和miRNA-146a表达的影响。方法:加味四逆散治疗二甲基亚硝胺(DMN)诱导的肝纤维化大鼠后,通过HE染色观察治疗前后肝组织病理变化,并通过RT-PCR技术检测miRNA-146a的表达。结果:与模型组比较,加味四逆散治疗组大鼠肝组织汇管区可见轻度炎症,纤维结缔组织增生程度较治疗前减轻,有显著性差异(P≤0.05)。治疗后的大鼠肝组织中miRNA-146a的表达明显升高。结论:加味四逆散科通过调节miRNA-146a的表达实现对肝纤维化的逆转。
Objective：To study the significance of pathological changes and miRNA‐146a expression in liver tis‐sue of Hepatic Fibrosis in Rats treated with Jiawei Sini powder .Methods ：The pathology changes were observed by HE staining and expressions of miRNA‐146a in liver tissue of hepatic fibrosis in rats treated with Jiawei Sini powder were de‐tected by SYBR Green quantitative reverse transcription polymerase chain reaction （ qRT‐PCR） analysis .Results：There were only mild inflammation in portal area and ease of fibrous connective and the expression of the miRNA‐146a signifi‐cantly increased in liver tissue of hepatic fibrosis rats treated with Jiawei Sini powder .Conclusion ：Jiawei Sini powder could regulate the expression of miRNA‐146a to inhibit hepatic fibrosis .
出处
《陕西中医》
2015年第3期371-373,共3页
Shaanxi Journal of Traditional Chinese Medicine
基金
咸阳市科学技术研究计划项目(2014k04-02)
