摘要
目的建立同时测定接骨丸中芍药苷、柚皮苷、橙皮苷、丹酚酸B、丹参酮ⅡA的HPLC-DAD方法。方法采用高效液相色谱波长切换法。Megres C18色谱柱（250 mm×4.6 mm,5μm）;以乙腈–0.1%磷酸溶液为流动相,进行梯度洗脱;检测波长切换为0～15 min为230 nm、15～45 min为280 nm、45～68 min为270 nm;体积流量1.0 mL/min;柱温30℃;进样体积10μL。结果芍药苷在35.27～352.70 ng、柚皮苷在8.625～86.250μg、橙皮苷在53.11～531.10μg、丹酚酸B在19.34～193.40μg、丹参酮ⅡA在2.142～21.420μg时进样量与峰面积积分值的线性关系良好。芍药苷、柚皮苷、橙皮苷、丹酚酸B、丹参酮ⅡA的平均回收率分别为100.47%、96.78%、97.45%、100.25%、99.94%,RSD值分别为0.48%、1.07%、1.11%、1.18%、1.83%。结论本法简便、快速、准确,可同时测定接骨丸中芍药苷、柚皮苷、橙皮苷、丹酚酸B、丹参酮ⅡA。
Objective To establish a quantification method for simultaneous determination of paeoniflorin, naringin, hesperidin, salvianolic acid B, and tanshinone ⅡA in Jiegu Pills by HPLC-DAD. Methods HPLC simultaneously with changing ultraviolet-visible wavelength was used. Megres C18 column（250 mm × 4.6 mm, 5 μm） was used with acetonitrile- 0.1% phosphoric acid solution as mobile phase in gradient elution mode. The detection wavelengths were set at 230 nm（retention time 0 — 15 min）, 280 nm（retention time 15 — 45 min）, and 270 nm（retention time 45 — 68 min）, respectively. Injection volume was 10 μL at the flow rate of 1.0 mL/min. The column temperature was set at 30 ℃. Results There were good linear relationships between peak areas and contents of five components, such as paeoniflorin, naringin, hesperidin, salvianolic acid B, and tanshinone ⅡA in the ranges of 35.27 — 352.70 ng, 8.625 — 86.250 ng, 53.11 — 531.10 ng, 19.34 — 193.40 ng, and 2.142 — 21.420 ng. The average recoveries of five components were 100.54%, 96.78%, 97.45%, 100.25%, and 99.94% with RSD values of 0.48%, 1.07%, 1.11%, 1.18%, and 1.83%, respectively. Conclusion The established method is convenient, accurate with a satisfactory separation, and high sensitivity. It can be used to determine paeoniflorin, naringin, hesperidin, salvianolic acid B, and tanshinone ⅡA in Jiegu Pills with the same chromatogram condition.
出处
《现代药物与临床》
CAS
2015年第2期153-156,共4页
Drugs & Clinic
