摘要
目的探讨微小 RNA（miRNA）miR-224在结肠癌组织中的表达，以及对体外癌细胞增殖和凋亡的影响。方法收集2011年2月至2014年3月行手术治疗的134例结肠癌和癌旁组织标本，利用实时荧光定量聚合酶链反应（Real-time PCR）检测标本中 miR-224表达情况；对人结肠癌细胞 SW480进行 miR-224反义寡核苷酸转染并培养，利用克隆形成实验和流式细胞仪检测 miR-224反义寡核苷酸对 SW480细胞增殖和凋亡的影响，利用 Real-time PCR 和 Western blot 法对不同转染组细胞中 Bcl-2表达情况进行检测。结果miR-224在结肠癌组织中相对表达水平显著高于癌旁组织（P 〈0.05）；反义 miR-224组人结肠癌 SW480细胞克隆形成率显著低于对照组，而细胞凋亡指数则高于对照组，差异均具有统计学意义（P 〈0.05）；反义 miR-224组细胞 Bcl-2相对表达量和蛋白相对表达量均低于对照组（P 〈0.05）。结论miR-224在结肠癌组织中呈高表达，减少 miR-224表达可抑制结肠癌细胞增殖，并加速细胞凋亡，有可能成为结肠癌早期发现及基因治疗的新靶位。
Objective To explore the expression of miR-224 in colon cancer and examine its effect on the proliferation and apoptosis of cancer cell.Methods A total of 134 cases of colon cancer and adjacent tissue specimens were collected from Feb.201 1 to Mar.2014.And the expression of miR-224 was detected by real-time polymerase chain reaction （PCR）.Human colon cancer cell SW480 was transfected by miR-224 antisense oligonucleotide and cultured.The effects of miR-224 antisense oligonucleotide on cellular proliferation and apoptosis were detected by colony formation assay and flow cytometry.And the expressions of Bcl-2 in differentially transfected cells were detected by real-time PCR and Western blot.Results The relative expression of miR-224 was significantly higher in colon cancer tissue than that in adjacent tissues.And the difference was statistically significant （P 〈0.05）. The colony formation rate of SW480 human colon cancer cell in antisense miR-224 group was signifi-cantly lower than that in control group while the apoptotic index was higher.And the differences were statistically significant （P 〈0.05）.The relative expressions of Bcl-2 mRNA and protein were lower in antisense miR-224 group than those in control group.And the differences were all statistically signifi-cant （P 〈0.05）.Conclusions There is an over-expression of miR-224 in colon cancer.And its down-regulation may effectively inhibit the proliferation and accelerate the apoptosis of colon cancer cell.It will probably become a new target for early detection and gene therapy of colon cancer.
出处
《腹部外科》
2015年第1期40-43,共4页
Journal of Abdominal Surgery
