摘要
目的建立同时测定九制大黄丸中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚含量的高效液相色谱（HPLC）法。方法色谱柱为C18柱（250 mm×4.6 mm,5μm）,流动相为甲醇-0.1%磷酸溶液（77∶23）,流速1.0 m L/min,检测波长254 nm,柱温40℃,进样量20μL。结果芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的进样质量浓度线性范围分别为4.6~92μg/m L,3.8~76μg/m L,4.0~800μg/m L,5.0~100μg/m L和17.6~352μg/m L,平均回收率分别为99.38%,99.04%,99.72%,99.59%和99.52%,RSD分别为0.80%,1.02%,0.50%,0.54%,0.92%（n=6）。结论 HPLC法可同时测定九制大黄丸中5组分的含量,专属性强,分离效果好。
Objective To establish a HPLC method for simultaneously determining the contents of aloe-emodin, chrysophanic acid,e modin, chrysophanol and rheochrysidin in Jiuzhi Rhubarb Pill. Methods The C18 column（ 250 mm × 4. 6 mm,5 μm） was adopted with methanol-0. 1% phosphoric acid（ 77 ∶ 23） as the mobile phase at a flow rate of 1. 0 m L / min,the detection wavelength was 254 nm,the column temperature was 40 ℃ and the injection volume was 20 μL. Results The linear ranges were 4. 6-92 μg / m L for aloe-emodin,3. 8-76 μg / m L for chrysophanic acid, 4. 0-800 μg / m L for emodin, 5. 0-100 μg / m L for chrysophanol and 17. 6-352 μg / m L for rheochrysidin respectively. The average recovery rates were 99. 38%（ RSD = 0. 80%）, 99. 04%（ RSD = 1. 02%）, 99. 72%（ RSD =0. 50%）,99. 59%（ RSD = 0. 54%） and 99. 52%（ RSD = 0. 92%） respectively（ n = 6）. Conclusion The HPLC method can simultaneously determine the contents of 5 kinds of components in Jiuzhi Rhubarb Pill and has stong specificity and good separating effects.
出处
《中国药业》
CAS
2015年第5期34-36,共3页
China Pharmaceuticals
