摘要
【目的】探讨不同证型甲状腺功能亢进症(甲亢)患者CD8+T细胞异常表达规律。【方法】收集阴虚型、阳虚型及阴阳两虚型甲亢患者各25例,以25例健康人作为正常对照组,采用流式细胞术检测外周血CD8+CD28+T细胞、CD8+CD28-T细胞及其胞内细胞因子白细胞介素-2(IL-2)、白细胞介素-4(IL-4),放射免疫法检测甲状腺功能指标如血清游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)和促甲状腺素(TSH)水平,并分析其相关性。【结果】除阴阳两虚组CD8+CD28-T细胞百分率和CD8+CD28+/CD8+CD28-比值外,阴虚、阳虚和阴阳两虚3证候组甲亢患者CD8+CD28+T细胞百分率、CD8+CD28-T细胞百分率、CD8+CD28+/CD8+CD28-比值、CD8+CD28+T细胞内IL-4百分率、CD8+CD28-T细胞内IL-2百分率与正常对照组比较,差异均有统计学意义(P<0.05)。3证候组组间比较差异均有统计学意义(P<0.05)。与正常对照组比较,阴虚组、阳虚组和阴阳两虚组甲亢患者FT3及FT4表达水平显著升高,TSH水平显著降低,3证候组组间比较差异均有统计学意义(P<0.05)。按CD8+CD28+T细胞及其胞内IL-4百分率、CD8+CD28+/CD8+CD28-比值和FT3、FT4表达水平高低,各组排列顺序为阴虚组>阴阳两虚组>正常组>阳虚组。按CD8+CD28-T细胞及其胞内IL-2百分率、TSH表达水平高低,各组排列顺序为阳虚组>正常组>阴阳两虚组>阴虚组。3证候组甲状腺功能3项指标与CD8+T细胞及其细胞因子均呈显著相关性(均|r|>0.7,P<0.05)。【结论】CD8+T细胞免疫异常在甲亢发病过程中具有重要作用。甲亢阴虚、阳虚、阴阳两虚分型与CD8+T细胞分布密切相关,阴虚组CD8+T细胞异常表达情况与阳虚组恰好相反。
Objective To explore the changes of CD8^＋T cells in the hyperthyroidism patients with different types of traditional Chinese medical syndrome. Methods Hyperthyroidism patients with yin deficiency type, yang deficiency type and yin-yang deficiency type were collected, 25 cases for each type. Additionally, 25 healthy volunteers served as normal control group. Flow cytometry was used to measure peripheral blood CD8^＋CD28^＋T cells, CD8^＋CD28^-T cells and intracellular cytokines such as interleukin（IL）-2 and IL-4. Radioimmunoassay was used to determine serum free triiodothyronine（ FT3）, free thyroxine（ FT4） and thyroid stimulating hormone（ TSH） levels. And then the correlation of thyroid functions with CD8^＋T cell percentages and their intracellular cytokines were compared and analyzed. Results Except CD8^＋CD28^-T cell percentage and CD8^＋CD28^＋/CD8^＋CD28^-ratio in yin-yang deficiency type, CD8^＋CD28^＋T cell percentage, CD8^＋CD28^-T cell percentage, CD8^＋CD28^＋/ CD8^＋CD28^-ratio, IL-4 percentage of CD8^＋CD28^＋T cells and IL-2 percentage of CD8^＋CD28^-T cells in hyperthyroidism patients with the three syndrome types were significantly different from those in the normal control group（ P〈0.05）, and the inter-group differences were significant in the three groups（ P〈0.05）. FT3 and FT4 levels were increased and TSH level was decreased in the hyperthyroidism patients with yin deficiehcy, yang deficiency and yin-yang deficiency syndrome types（P〈0.05 compared with the normal control group）, the inter-group differences being significant in the three groups（P〈0.05）. The decreasing listing order was yin deficiency group, yin-yang deficiency group, normal control group, yang deficiency group with reference to CD8^＋CD28^＋T cell percentage, IL-4 percentage of CD8^＋CD28^＋T cells, CD8^＋CD28^＋/ CD8^＋CD28^-ratio, and FT3 and FT4 levels. When with reference to CD8^＋CD28^-T cell percentage, IL-2 percentage of CD8^＋CD28^-T cells,and TSH level, th
出处
《广州中医药大学学报》
CAS
2015年第2期194-198,共5页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
韶关市卫生局资助项目(编号:WSTJJ201311022430521197807197088)
