摘要
在单因素实验的基础上,利用Box-Behnken中心组合实验设计及响应面(RSM)分析,研究三种复合生物保鲜剂对南美白对虾冷藏保鲜效果的影响。以冷藏6d的南美白对虾细菌总数为考核指标,并采用Design-Expert软件对复合生物保鲜剂与细菌总数之间建立的二次回归模型进行分析,获得三种复合生物保鲜剂的最优浓度组合。结果表明:三种复合生物保鲜剂的最佳使用浓度为Nisin 0.04g/100m L、ε-PL 0.48g/100m L和TP 1.06g/100m L,细菌总数理论值为1.81×103CFU/g,三次平行验证实验细菌总数最多为1.98×103CFU/g,误差仅为9.39%,说明模型合适可信,可以用来分析和预测南美白对虾冷藏保鲜期间的细菌总数。该复合生物保鲜剂可以相互补充,协同抗菌,有效延长南美白对虾的货架期。
On the basis of single factor experiment,the aim was to study the influence of three kinds of compound biological preservative on effect of cold preservation of P. vannamei through the analysis of Box-Behnken central composite design and response surface methodology（RSM）. Using the total number of bacteria of P. vannamei which had been cold stored for 6 days as evaluation index,and using Design-Expert software to analyze the quadratic regression model of compound biological preservatives and the total number of bacteria, and then optimal concentration combinations of three kinds of compound biological preservative were obtained. The results showed that the optimal concentration of the three kinds of compound preservative were Nisin 0.04g/100mL,ε-PL 0.48g/100mL and TP 1.06g/100mL. The total number of bacteria was 1.81 ×10^3CFU/g. The largest number was 1.98×10^3FU/g in the three parallel verification of total bacteria tests,error was only 9.39%, which meant the model was appropriate and credible. It could be used to analyze and predict the total number of bacteria of the P. vannamei when it was cold stored. The compound biological preservative could be mutually complementary and synergistic antibacterial,which could extend the shelf life of P. vannamei effectively.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第6期337-341,350,共6页
Science and Technology of Food Industry
基金
国家自然科学基金项目(31070701)
关键词
南美白对虾
复合生物保鲜
响应面优化
Penaeus vannamei
composite biological preservation
RSM optimization
