摘要
采用EGCG单体对斑点叉尾鮰鱼皮胶原薄片进行改性。从斑点叉尾鮰鱼皮中提取了胶原之后,利用EGCG分别对胶原薄片和胶原溶液进行了交联。然后利用DSC、FITR、X-RAY和SEM分析了胶原薄片改性前后的性质变化,并与戊二醛改性的胶原薄片进行了对比。结果表明,胶原薄片先成型再利于EGCG改性,EGCG浓度为0.075%时,使胶原薄片的玻璃态转化温度从194℃提高到207℃,并将胶原晶面间距离从12.6373nm减小到11.9422nm,大幅度拉近了肽链之间的距离,使肽链间连接更紧密。对比未改性胶原薄片,EGCG改性样品截面紧密多空。本研究表明,EGCG可以用于胶原改性。本研究为活性天然产物用于胶原改性提供了有用信息。
In order to get nontoxic crosslinking collagen sheet, EGCG was added to channel catfish skin collagen. Firstly,fish collagen was extracted from channel catfish skin. Subsequently,EGCG was added to collagen solution and collagen sheet. Physical properties and microstructural changes in the samples were analyzed using DSC,FTIR,XRD and SEM. Difference between collagen sheets modified by EGCG and glutaraldehyde were analyzed. The results indicated that EGCG could be used a modifier of formed collagen sheet. The glass transition temperature of collagen sheet by 0.075% EGCG was improved from 194℃ to 207℃. Simultaneously, the corresponding d value that represented the basal spacing of the samples decreased considerably from 12.6373 to 11.9422nm because of the EGCG added. The section of collagen sheets modified by EGCG was more compact compared to control. In conclusion,EGCG could be used to modify collagen sheet. This study provided some useful information for collagen modification by natural active substance.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第6期99-103,共5页
Science and Technology of Food Industry
基金
中央高校基本科研业务费专项资金资助(JUSRP21010)
关键词
鱼皮胶原
EGCG
交联
性质
玻璃态转化温度
fish skin collagen
EGCG
crosslinking
properties
glass transition temperature
