摘要
目的 观察Math1（mouse atonal homologue 1 gene,Math1）基因及神经生长因子β基因（nerve growth factor beta gene,NGFβ）共转染对噪声性听力损伤豚鼠耳蜗螺旋神经节细胞的保护作用.方法 选用40只健康成年白色纯种豚鼠制备稳态噪声（110 dB SPL）耳聋模型,任选爆震前后听阈阈移大于60 dB SPL的动物作为实验动物,共选35只,采用数字表法随机分为4组,其中Ⅰ组10只为双基因组,导入腺病毒介导的双基因Ad-Math 1/NGFβ;Ⅱ组10只为Math1组,导入腺病毒介导的单基因Ad-Math1;Ⅲ组10只为NGF组,导入腺病毒介导的单基因Ad-NGFβ;Ⅳ组5只为对照组,导入腺病毒;另选健康成年白色纯种豚鼠5只为正常组,不接受噪声和基因转染.基因转染后第1周,取豚鼠耳蜗组织固定切片行免疫组织化学染色;基因转染后第4周,取豚鼠耳蜗组织固定切片行HE染色.结果 基因转染后1周,Ad-Math 1/NGFβ在豚鼠耳蜗内成功转染,耳蜗各回膜性组织中均有表达,且表达强度基本相等;基因转染后第4周HE染色显示：Ⅰ组豚鼠耳蜗组织中螺旋神经节细胞数平均为（166.4 ±2.58）个,明显多于Ⅱ组（92.1±1.34）个、Ⅲ组（88.6±1.22）个,差异均有统计学意义（P＜0.01）,且细胞形态与正常细胞形态相近.结论 Math1联合NGFβ基因共转染在噪声损伤后豚鼠耳蜗中高效表达,对噪声性听力损伤豚鼠耳蜗螺旋神经节细胞有明显的保护作用.
Objective To observe the protective effect of Math1/NGFβ （Mouse atonal homologue Ⅰ gene/ nerve growth factor beta gene） gene transfection on spiral ganglion cells in guinea pigs with noise-induced hearing damage.Methods Forty heahhy white guinea pigs were used to develop the model of stable noiseinduced hearing damage （110 dB SPL）.Thirty-five animals with hearing threshold greater than 60 dB SPL both before and after burst explosion were chosen as experimental animals,and then were randomly divided into 4 groups：groups Ⅰ,Ⅱ,Ⅲ and Ⅳ.The animals in group Ⅰ （n =10） （or the Mathl/NGFβ group） were inoculated with Ad-Math1/NGFβ,the animals in group Ⅱ （n =10） or （the Math1 group） were imported with Ad-Mathl,the animals in group Ⅲ（n =10） （or the NGF group） were imported with Ad-NGFβ,the animals in group Ⅳ （or the control group） were imported with adenovirus,and still another 5 healthy white guinea pigs were chosen as the normal group,which had neither noise exposure nor gene transfection.Cochlea sections of guinea pigs were collected for immunohistochemical staining,respectively at week 1 after gene transfection.Then,at week 4 after gene transfection,cochlea sections of guinea pigs were collected for HE staining.Results At week 1 after gene transfection,genes of Ad-Math1/NGFβ were successfully transfected in the cochlea of guinea pigs and expressions of Ad-Math1/NGFβ protein could be detected in each turn of the cochlea,with expression intensity being essentially the same.HE staining at week 4 after gene transfection indicated that the number of spiral ganglions in the Math1/NGFβ group was on the average （166.4 ± 2.58）,which was significantly greater than those of group Ⅱ （92.1 ± 1.34） and group Ⅲ （88.6 ± 1.22））,with statistical significance （P 〈 0.01）.Conclusions Ad-Math1/NGFβ could be expressed at a high level in the noiseimpaired cochlea,suggesting that Ad-Math1/NGFβ seemed to have a protective effect on cochlear spiral ganglion
出处
《中华航海医学与高气压医学杂志》
CAS
CSCD
北大核心
2014年第3期163-167,共5页
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
基金
全军医药卫生“十二五”科研基金面上课题（CWS11J128）
全军医药卫生“十二五”科研基金重点课题（BWS11C035）
