摘要
目的探讨分析冠心病(CHD)合并糖尿病(DM)患者糖基化终产物(AGEs)与单核细胞toll样受体4(TLR4)的关系。方法 CHD+DM患者58例(CHD+DM组),CHD患者60例(CHD组),健康对照组50例。采用酶联免疫吸附测定(ELISA)法检测各组的AGEs,用流式细胞仪检测外周血单核细胞TLR4m RNA相对表达量及TLR4阳性单核细胞比率。结果与健康对照组比较,CHD+DM组及CHD组AGEs及外周血单核细胞TLR4m RNA相对表达量、TLR4阳性单核细胞比率明显增高(P<0.05);CHD+DM组与CHD组比较,CHD+DM组AGEs及外周血单核细胞TLR4m RNA相对表达量、TLR4阳性单核细胞比率增高(P<0.05);相关性分析显示升高的AGEs与升高的TLR4m RNA相对表达量及TLR4阳性单核细胞比率呈正相关。结论 CHD+DM患者AGEs及TLR4表达高于CHD患者;CHD患者AGEs及TLR4表达高于健康对照组。AGEs与TLR4的表达呈正相关。
Objective To investigate the relationship between advanced glycation end products（AGEs） and monocyte Toll-like receptor 4（TLR4） in patients with coronary heart disease（CHD） combined with diabetes mellitus（DM）. Methods There were 58 patients with CHD＋DM（CHD＋DM group）, 60 patients with CHD only（CHD group）, and 50 patients in healthy control group. Enzyme-linked immuno sorbent assay（ELISA） was applied to detect AGEs in all the groups, and flow cytometry was used to detect relative expression of peripheral blood monocytes TLR4 m RNA and ratio of TLR4 positive monocytes. Results Compared with the healthy control group, CHD＋DM group and CHD group all had remarkably increased AGEs, relative expression of peripheral blood monocytes TLR4 m RNA, and ratio of TLR4 positive monocytes（P0.05）. Between the CHD＋DM group and CHD group, the former had higher increased levels of AGEs, relative expression of peripheral blood monocytes TLR4 m RNA, and ratio of TLR4 positive monocytes（P0.05）. The results of correlated analysis showed that there was positive correlation between increased AGEs, increased relative expression of TLR4 m RNA, and ratio of TLR4 positive monocytes. Conclusion The expressions of AGEs and TLR4 of CHD＋DM patients are higher than CHD patients, and those of CHD patients are also higher than healthy control group. AGEs and expression of TLR4 are positively correlated.
出处
《中国实用医药》
2015年第4期1-2,共2页
China Practical Medicine
基金
沈阳市科技计划项目(项目编号:F11-262-9-18)
关键词
冠心病
糖尿病
糖基化终产物
TOLL样受体4
Coronary heart disease
Diabetes mellitus
Advanced glycation end products
Toll-like receptor 4
