摘要
目的:观察凉血解毒汤对银屑病小鼠皮肤组织中CC趋化因子配体20(CCL20)/CC趋化因子受体6(CCR6)表达的干预作用。方法:24只BALB/c雄性小鼠随机分为空白对照组、银屑病模型组、凉血解毒汤中药治疗组和趋化因子CCL20单克隆抗体(阳性药)治疗组。用咪喹莫特诱导小鼠银屑病模型,采用银屑病皮损面积和疾病严重程度(psoriasis area and severity index,PASI)评分标准观察银屑病样小鼠皮损的变化情况。光镜下观察皮损组织形态学变化,测量表皮层厚度。采用real-time PCR检小鼠皮肤组织样本中CCL20和CCR6表达的变化。结果:银屑病模型组小鼠皮肤出现大量鳞屑和红斑,表皮增厚;与银屑病模型组小鼠比较,凉血解毒汤组小鼠银屑病样皮损程度较轻,红斑、鳞屑以及表皮增厚程度轻于模型组,PASI分数降低,皮肤组织CCL20以及CCR6的表达明显低于模型组。趋化因子CCL20单克隆抗体治疗组小鼠银屑病皮损程度较轻,红斑、鳞屑以及表皮增厚程度轻于模型组,PASI分数降低,皮肤组织CCL20以及CCR6的表达明显低于模型组。结论:凉血解毒汤可能通过下调CCL20/CCR6的表达来减轻银屑病小鼠的皮肤病变。
AIM： To observe the effects of Liangxue-Jiedu（ LXJD） prescription,a Chinese medicine,on the expression of CC chemokine ligand 20（ CCL20） and CC chemokine receptor 6（ CCR6） in imiquimod-induced psoriasislike skin lesions in a mouse model. METHODS： Male BALB / c mice were randomly divided into control group,model group,LXJD treatment group and chemokine CCL20 monoclonal antibody treatment group. The mouse model of psoriasis was induced by imiquimod. The severity of psoriasis was assessed by the dermatologists using psoriasis area and severity index（ PASI）. The morphological changes of the skin tissues were observed under light microscope. The thickness of epidermis was measured. Real-time PCR was used to detect the expression of CCL20 and CCR6 in the skin tissue samples. RESULTS： The skin in model group showed a large number of scales,erythema and skin thickening. Compared with model group,the skin lesion in LXJD treatment group was attenuated. The erythema and scales were alleviated,the PASI score was reduced,and the expression of CCL20 and CCR6 was significantly lower than that in model group. CONCLUSION：LXJD prescription down-regulates the expression of CCL20 / CCR6,thus attenuating the psoriasis skin lesions in mice.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2015年第2期331-336,共6页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.81302985)
