摘要
目的观察小鼠髓源性树突状细胞(DC)在舒尼替尼刺激下,其表面程序性死亡分子1配体1(PD-L1)和PD-L2的表达变化。方法取小鼠骨髓细胞,对照组加入二甲基亚砜,实验组分别加入(100、200、300)ng/m L舒尼替尼,刺激48 h,用流式细胞术检测DC表面PD-L1和PD-L2的表达水平。结果与对照组相比,实验组中成熟DC(m DC)和总DC(包括m DC和im DC)表面PD-L1的表达水平显著降低;表达PD-L1的未成熟DC(im DC)、m DC和DC百分比均显著降低,表达PD-L2的m DC百分比显著降低;表达PD-L2的DC百分比在100 ng/m L、300 ng/m L舒尼替尼组中显著降低。结论舒尼替尼可显著降低小鼠DC表面PD-L1、PD-L2的表达。
Objective To observe the changes of programmed death-1 ligand 1 （PD-L1） and PD-L2 expressions on mouse bone marrow-derived dendritic ceils （DCs） stimulated by sunitinib. Methods DCs were randomly divided into four groups which were treated with sunitinib （100, 200, 300 ng/mL） and dimethylsulfoxide （DMSO）, respectively. After 48 hours, PD-L1 and PD-L2 expression levels were analyzed by flow cytometry. Results Compared with the control group, the expression of PD-L1 on mature DCs （mDCs） and all DCs[ including mature DCs and immature DCs （imDCs） ] was significantly down-regulated in sunitinib treatment groups. The PD-L1 expression percentages of imDCs, mDCs and DCs were significantly reduced in sunitinib treatment groups; the percentage of mDCs expressing PD-L2 also dropped in all treatment groups, and the percentage of DCs expressing PD-L2 decreased in 100 and 300 ng/mL sunitinib treatment groups. Conclusion Sunitinib can significantly reduce the expressions of PD-L1 and PD-L2 on mouse DCs.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2015年第1期10-13,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
吴阶平医学基金(32067521203)
