摘要
为了解聚糖菌在污泥颗粒化中的脱氮能力及其微生物生态特性,采用反应器工艺、批式试验、显微技术和荧光原位杂交技术来评估其反硝化能力,揭示其微生物群落的微观结构,探索聚糖菌和聚磷菌在不同粒径污泥中的分布特征.结果表明,污泥对有机物的吸收率稳定在90%以上.颗粒污泥的沉降指数(SVI10)稳定在30-50 mL g-1,远低于接种污泥的108.2 mL g-1.聚醣菌颗粒污泥对硝酸盐氮和亚硝酸盐氮的反硝化去除率分别达到了65%和70%,氮气为主要气态产物.聚糖菌颗粒污泥被大量的四联球菌结构所充斥,丝状菌在其中起到了重要的构架和搭桥的作用.荧光原位杂交结果表明聚糖菌可生存于各尺寸颗粒化污泥内;而聚磷菌受到严重抑制只能较少地分布在颗粒污泥的外围空间.上述结果表明,在SBR反应器中采用厌氧搅拌—排水—好氧曝气的处理模式成功培育出具有聚糖特性的颗粒化微生物聚集体,培育成功的颗粒污泥对硝酸盐氮和亚硝酸盐氮均具有良好的反硝化特性.在整体颗粒污泥微生物群落中聚糖菌为优势种群.
This study aimed to investigate the denitrification ability and microbial ecological characteristic of glycogenaccumulating organisms（GAOs） during sludge granulation. A combined method of reactor process, batch test, microscale techniques and fluorescence in situ hybridization（FISH） was employed to evaluate the denitrification ability of GAOs, to reveal the microstructure of microbial communities and to characterize the distribution of GAOs and polyphosphate-accumulating organisms（PAOs） in different size granules. The results indicated that the adsorption rate of organic compound by sludge was over 92%. The SVI10 of granules stabilized at 30-50 mL g-1, a very low value compared to those measured for inoculating sludge（108.2 mL g-1）. The nitrogen removal rate of nitrate and nitrite by GAOs granules reached 65% and 70% respectively, with N2 as the main gaseous product. The granules were tightly packed with a large amount of tetrad-forming organisms（TFOs）, with filamentous bacteria serving as the supporting structures for the granules. FISH demonstrated that GAOs were distributed evenly throughout the granular space, whereas PAOs were severely inhibited and presented only in the edge of granules. A labscale sequencing batch reactor（SBR） could be successfully operated for cultivating GAOs granules in alternating anaerobic stirring-discharge-aerobic modes, with the GAOs granules showing excellent denitrifying capability. Therefore GAOs are the predominant member of the microbial population in granules.
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2014年第4期551-557,共7页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金资助项目(51108456
50908227)
国家863计划项目(2009AA06Z312)资助~~
关键词
聚糖菌
反硝化
颗粒污泥
微生物群落动态
生物脱氮
气态产物
四联球菌
glycogen-accumulating organisms（GAOs）
denitrification
granular sludge
microbial community development
biological nitrogen removal
gaseous product
tetrad-forming organisms（TFOs）
