摘要
目的研究桑螵蛸挥发油提取物对耐甲氧西林金黄色葡萄球菌(MRSA)的体外抑菌效应。方法采用索氏提取法提取桑螵蛸中挥发油成分,在鉴定耐药基因基础上,再采用纸片扩散法(K-B法)、试管稀释法(MIC法)、吸光度值测定法来观察桑螵蛸挥发油提取物对MRSA的体外抑菌效应。结果桑螵蛸挥发油提取物对MRSA的生长表现出抑制效应。结论桑螵蛸挥发油提取物对MRSA具有一定的抑菌效应,为下一步研究其详细有效成分及开发提供数据和理论依据。
Objective To study the bacteriostatic effect of volatile oil from ootheca mantidis on methicillin-resistant Staphylococcus aureus（MRSA） in vitro. Methods The components of volatile oil from ootheca mantidis were extracted by Soxhlet extraction method,and antibacterial effect of the volatile oil extracts on MRSA were observed through Kindy-Bauer method,MIC method and absorbance assays after mecA gene of MRSA was identified by PCR. Results The volatile oil from ootheca mantidis exhibited bacteriostatic effect on the growth of MRSA.Conclusion The volatile oil from ootheca mantidis has antibacterial effect on MRSA,which provide the data and theoretical basis for further research and development of the active ingredients in ootheca mantidis.
出处
《中国微生态学杂志》
CAS
CSCD
2014年第8期874-877,共4页
Chinese Journal of Microecology
基金
辽宁省教育厅一般科研项目基金(L2012330)
关键词
桑螵蛸
挥发油
耐甲氧西林金黄色葡萄球菌
耐药性
Ootheca mantidis
Volatile oil
Methicillin-resistant staphylococcus aureus
Drug resistance
