摘要
目的建立白薇中蔓生白薇苷A及白薇C21甾体总皂苷的含量测定方法,并测定其在不同产地蔓生白薇中的含量。方法以蔓生白薇苷A为指标,采用高效液相色谱法和可见分光光度法分别对蔓生白薇苷A及白薇C21甾体总皂苷进行含量测定。结果蔓生白薇苷A和总皂苷含量分别在0.23-2.53μg（r=0.999 7）及11.5-115μg（r=0.999 1）内线性关系良好,平均回收率分别为99.3%（RSD=2.68%）和99.7%（RSD=2.84%）。蔓生白薇苷A的含量以河南最高,山东最低;白薇C21甾体总皂苷则以山东最高,辽宁最低。结论此方法简便、稳定可靠,便于对白薇药材进行质量评价。
OBJECTIVE To establish the method for the determination of cynanversicoside A and total C21-steroidal-glycoside in Cynanchi Atrati Radix from different areas. METHODS With the content and absorbance of cynanversicoside A as index, HPLC and UV were used to determine the contents of cynanversicoside A and total C21-steroidal-glycoside in all samples. RESULTS The linear range of cynanversicoside A and steroidal-glycoside was 0.23-2.53 μg and 11.5-115 μg, respectively, with the correlation coefficient of 0.999 7 and 0.999 1; the average recoveries were 99.3% and 99.7%, with RSD of 2.68% and 2.84%. There was great difference of the samples, content of cynanversicoside A of Henan was the highest, lowest in Shandong; the total C21-steroidal-glycoside in sample from Shandong was the highest, lowest in Liaoning. CONCLUSION The method is simple, accurate and reliable for the quality control of Cynanchi Atrati Radix.
出处
《中国现代应用药学》
CAS
CSCD
2014年第10期1228-1231,共4页
Chinese Journal of Modern Applied Pharmacy
基金
湖州市自然科学资金项目(2013YZ13)
关键词
白薇
C21甾体皂苷
蔓生白薇皂苷A
含量测定
Cynanchi Atrati Radix
C21-steroidal-glycoside
cynanversicoside A
content determination
