摘要
为提高红曲色素的液态发酵水平,降低生产成本,采用Plackett-Burman试验与Box-Benhnken试验对红曲霉MY-03液态发酵生产红曲色素的工艺条件进行了优化,获得了最佳工艺条件:葡萄糖50 g/L、蛋白胨58.07 g/L、Mg SO4 2 g/L、Na NO3 2g/L、Mn SO4 0.3 g/L、Zn SO4 0.1 g/L、装瓶量50.8 m L、接种量10.0%(V/V),于150 r/min、30℃恒温培养7 d,红曲色素色价可达342.24±2.88 U/m L,比基础培养基提高了86.9%。
In order to improve the yield of Monascus pigment and reduce the production costs, Monascus purpureus MY-03 was adopted to produce Monascus pigment in the liquid state fermentation, and the fermentation conditions were optimized by Plackett-Burman design and Box-Benhnken experimental design. The results showed that the optimum medium was composed of glucose 50 g/L, peptone 58.07 g/L, MgSO4 2 g/L, NaNO3 2 g/L, MnSO4 0.3 g/L, ZnSO4 0.1 g/L;and the optimum bottle load was 50.8 mL in a 250 mL flask, and the optimum inoculum volume was 10.0%（V/V）. The color value of Monascus pigment reached to 342.24±2.88 U/mL, increased by 86.9%than that in the basal medium, when the test fungi were incubated in 150 r/min and 30℃for 7 d.
出处
《生物技术通报》
CAS
CSCD
北大核心
2015年第2期187-195,共9页
Biotechnology Bulletin
关键词
红曲霉
液态发酵
红曲色素
响应面法
Monascus purpureus
submerged fermentation
Monascus pigment
Response Surface Methodology
