摘要
植物蓝光受体向光素(phototropin,PHOT)介导许多生理反应,现已从拟南芥中分离了其下游的一些信号转导组分。前期研究表明,拟南芥光敏色素底物PKS家族成员PKS1与部分Ca2+结合蛋白钙调素(calmodulin,CAM)成员互作,参与PHOT2介导的强蓝光诱导下胚轴向光反应。旨在探讨PKS2和CAM4之间的互作关系,首先用RT-PCR技术得到PKS2和CAM4的c DNA全长序列。通过酵母双杂交和双分子荧光互补技术,从体外与体内证实PKS2和CAM4能相互作用。此结果进一步丰富了PKS家族与CAM之间的联系,为深入解析PHOT功能研究奠定基础。
Blue-light receptors phototropin（PHOT）mediate a wide set of physiological and developmental responses. As some PHOT downstream signal transduction components have been identified in Arabidopsis by genetic analyses, we have found that PKS1 of Phytochrome kinase substrate PKS family interacts with members of calmodulin（CAM）, which are Ca2＋binding protein. In order to address the issue of how PKS2 interacts with CAM4, firstly cDNA full length sequences of PKS2 and CAM4 were obtained by RT-PCR technique. Then, it was confirmed that PKS2 could be interact with CAM4 by tests of yeast two-hybrid system and bimolecular fluorescence complementation. These date could contribute to enrich relation between PKS and CAM, which would provide base for further uncovering PHOT functions.
出处
《生物技术通报》
CAS
CSCD
北大核心
2015年第2期91-96,共6页
Biotechnology Bulletin
关键词
拟南芥
向光素
光敏色素底物
钙调素
Arabidopsis thaliana
phototropin
phytochrome kinase substrate
calmodulin
