摘要
目的建立高效液相色谱-电感耦合等离子体质谱联用技术（HPLC—ICP—MS）法测定单味雄黄和六神丸可溶性砷的含量方法；考察六神丸配伍对雄黄中可溶性砷溶出度的影响，探讨六神丸的配伍机制。方法分别建立了ICP-MS和HPLC—ICP—MS法测定雄黄及六神丸中总砷和可溶性砷含量的方法，采用人工胃液和人工肠液为溶出介质，考察雄黄和六神丸中可溶性砷的溶出情况，分析配伍使用对雄黄中可溶性砷溶出的影响。结果5种不同形态砷标准物质在5～500μg·L-1内线性关系良好，精密度和重复性均良好，回收率89％～102％。可溶性砷从六神丸复方中的溶出量和溶出速率均明显低于从单味雄黄中的溶出。结论六神丸复方配伍可显著降低雄黄中可溶性砷的溶出，从而降低其毒性。
AIM To establish the method for the determination of soluble arsenic in Liushen pills and regular by HPLC-ICP-MS and study the effect of other components in Liushen pills to the dissolution of arsenic. METHODS Total arsenic was determined by using microwave digestion and [CP-MS, and soluble arsenic was assayed by HPLC-ICP-MS. The dissolution rates of soluble arsenic from Liushen pills and regular were measured by taking pH 1.2 PBS and pH 6.8 PBS as media continuously. RESULTS The determination method had a good linearity, precision and recovery in the concentration range of 5 - 500 μg· L- 1 of five arsenic species. The dissolution rate of soluble arsenic from Liushen pills was significantly lower than that from realgar. CONCLUSION The other components in Liushen pills might reduce the dissolution of soluble arsenic from realgar and thus decrease the toxicity of it.
出处
《中国临床药学杂志》
CAS
2015年第1期17-22,共6页
Chinese Journal of Clinical Pharmacy
基金
上海市科学技术委员会中药现代化专项
传统名优中成药六神丸的二次开发(编号10DZ1970100)
