摘要
目的:探讨辛伐他汀对前列腺上皮细胞RWPE-1增殖及凋亡的影响。方法:设定不同浓度的辛伐他汀(0、10、20、40μmol/L)分别作用于体外培养的RWPE-1细胞,利用MTT法检测细胞增殖情况,流式细胞术检测细胞的凋亡情况。荧光定量RT-PCR检测RWPE-1细胞的Bcl-2、Bax、Cx43 mRNA的表达,Western印迹检测Bcl-2、Bax、Cx43蛋白的表达。结果:MTT法检测辛伐他汀(10、20、40μmol/L)作用于RWPE-1细胞72 h后,对RWPE-1细胞的抑制率分别为(21.07±6.41)%、(34.87±9.65)%和(47.18±10.88)%,与对照组[(1.21±0.54)%]比较有显著差异(P<0.05),并且呈明显的剂量依赖关系(P<0.05);处理72 h后各组的凋亡指数分别为:10μmol/L组(0.066±0.016)%,20μmol/L组(0.126±0.023)%,40μmol/L组(0.192±0.025)%,与对照组[(0.015±0.005)%]相比差异显著(P<0.01),且呈剂量依赖关系(P<0.05)。荧光定量PCR检测显示随着辛伐他汀浓度升高Bcl-2基因表达逐渐下调(P<0.05),Bax和Cx43基因表达逐渐上调(P<0.05),并且呈剂量依赖关系(P<0.05)。Western印迹检测显示RWPE-1细胞内Bcl-2蛋白的表达随辛伐他汀浓度的增高逐渐下调(P<0.05),Bax蛋白逐渐上调(P<0.05),Cx43蛋白逐渐上调(P<0.01),并且皆呈剂量依赖关系(P<0.05)。Cx43的表达与Bcl-2的表达呈负相关,与Bax的表达呈正相关。结论:辛伐他汀可能通过影响细胞间隙连接通讯来抑制前列腺上皮细胞增殖并诱导其凋亡。
Objective： To investigate the effects of simvastatin on the proliferation and apoptosis of prostatic epithelial RWPE-1 cells. Methods： RWPE-1 cells cultured in vitro were treated with simvastatin at0, 10, 20, and40 μmol/L for 24, 48, and 72 hours followed by determination of their proliferation by MTT assay, and their apoptosis by flow cytometry. The mRNA and protein expressions of Bcl-2, Bax, and Cx43 were detected by fluorescence quantitative RT-PCR and Western blot, respectively. Results ： After 72 hours of treatment with simvastatin at 10, 20, and 40 μmol/L, the inhibition rates of the RWPE-1 cells were （21.07 ± 6.41 ）% , （ 34.87 ± 9.65 ） %, and （47.18 ± 10.88 ） %, respectively, significantly higher than （ 1.21 ±0.54 ） % in the control group （ P 〈 0.05 ） and in a dose-dependent manner （ P 〈 0.05 ） ; the cell apoptosis rates were （0. 066 ± 0. 016 ） % , （0. 126 ± 0. 023 ） %, and （0. 192 ±.025）% , respectively, remarkably higher than （0.015 ± 0. 005）% in the control （P 〈 0.05） and also in a dose-depend-ent manner （ P 〈 0.05 ） ; the mRNA and protein expressions of Bcl-2 were decreasing while those of Bax and Cx43 increasing with the increased concentration of simvastatin （P 〈 0.05）. The expression of Cx43 was correlated negatively with that of Bcl-2 but positively with that of Bax. Conclusion ： Simvastatin inhibits the proliferation of prostate epithelial cells and induce their apoptosis by acting on the gap junctional intercellular communication.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2015年第2期113-118,共6页
National Journal of Andrology
基金
福建省自然科学基金(2012J05164)~~
