摘要
目的:探讨左卡尼汀对糖尿病心肌病模型大鼠心肌细胞凋亡的影响。方法:采用高脂高糖饲料加腹腔注射链脲佐菌素建立糖尿病心肌病大鼠模型,随机分为模型(生理盐水)组和左卡尼汀低、中、高剂量(30、40、50 mg/d)组,另取正常大鼠作为正常对照(纯净水)组,每组10只,ig给予相应药物,每天1次,连续6周。末次给药后检测各组大鼠心室内压、心肌细胞凋亡指数和心肌细胞中B淋巴细胞瘤2(Bcl-2)、Bcl-2相关X蛋白(Bax)、p53基因蛋白的表达。结果:与正常对照组和模型组比较,左卡尼汀低、中、高剂量组大鼠的心率、左心室收缩压、左心室内压最大上升和下降速率均降低,左心室舒张末期压升高,差异具有统计学意义(P<0.01);与模型组比较,左卡尼汀低、中、高剂量组大鼠的心肌细胞凋亡指数、Bax和p53基因蛋白的表达均明显降低,Bcl-2基因蛋白的表达明显升高,差异具有统计学意义(P<0.01或P<0.05),其中以左卡尼汀中剂量组效果最显著。结论:左卡尼汀对糖尿病心肌病模型大鼠心肌细胞的凋亡有抑制作用。
OBJECTIVE： To study the effect of L-carnitine on cardiac cell apoptosis of diabetic cardiomyopathy rats. METH- ODS： Diabetic cardiomyopathy model rats were established by using high-lipid high-sugar forage and intraperitoneal injection of streptozotocin, then randomly divided into model group （normal saline）, L-carnitine low, medium and high dose groups （30, 40, 50 mg/d） and normal control group （pure water）, 10 for each with drug administration for 6 weeks, once a day. Rat ventricular pressure, cardiac myocyte apoptosis index, and protein expression of B-cell lymphoma 2 （Bcl-2）, Bax, p53 gene were determined after the last administration. RESULTS： Compared with normal control group and model group, the heart rate, left ventricular sys- tolic blood pressure , maximum rising and falling rate of left heart indoor pressure were decreased, and the left ventricular end-dia- stolic pressure was increased in L-carnitine treatment group, there was significant difference （P〈0.01）. Compared with model group, apoptosis index, Bax and protein expressions of p53 gene was significantly decreased, and exprotein pression of Bcl-2 gene was significantly increased in L-carnitine treatment group （P〈0.01 or P〈0.05）, especially significant in the L-carnitine medium dose group. CONCLUSIONS ： The L-camitine can suppress the cardiac cell apoptosis of diabetic cardiomyopathy rats.
出处
《中国药房》
CAS
北大核心
2015年第7期933-935,共3页
China Pharmacy
