摘要
目的:研究木犀草素对模型小鼠癌性恶病质的改善作用。方法:皮下接种小鼠结肠腺癌细胞Colon26于雄性BALB/C小鼠,以复制小鼠癌性恶病质模型。将40只BALB/C小鼠随机均分为正常对照(等容生理盐水)组、模型(等容生理盐水)组与木犀草素高、低剂量(100、50 mg/kg)组,ig给药,每天1次,连续7 d。处死小鼠称定小鼠净质量、干质量、腓肠肌质量、附睾脂肪质量、全身脂肪质量和肿瘤质量;采用自动生化分析仪检测小鼠血液甘油三酯(TG)、非酯化脂肪酸(NEFA)、总胆固醇(TC)和葡萄糖含量;采用荧光法测定小鼠腓肠肌中蛋白酶和钙激活蛋白酶活性;采用酶联免疫(ELISA)法检测小鼠血清中肿瘤坏死因子α(TNF-α)与白细胞介素6(IL-6)的水平。结果:与正常对照组比较,模型组小鼠净质量、干质量、腓肠肌质量、附睾脂肪质量、全身脂肪质量减少,肿瘤质量增加;TG、NEFA、TC含量增加,葡萄糖含量减少;小鼠腓肠肌中泛素蛋白酶和钙激活蛋白酶活性增强;小鼠血液IL-6含量增加,差异均有统计学意义(P<0.01或P<0.05)。与模型组比较,木犀草素高、低剂量组小鼠净质量、干质量、腓肠肌质量、附睾脂肪质量增加;TC、TG、NEFA含量减少,葡萄糖含量增加;小鼠腓肠肌中泛素蛋白酶和钙激活蛋白酶活性减弱;小鼠血液IL-6含量减少;木犀草素高剂量组小鼠全身脂肪质量增加,差异均有统计学意义(P<0.05)。结论:木犀草素可改善模型小鼠癌性恶病质症状,其机制可能与抑制蛋白酶、钙激活蛋白酶活性和降低细胞因子的水平有关。
OBJECTIVE： To study the effect of luteolin on the improvement of cancerous cachexia in model mice. METH- ODS： Murine colonic cancer cells Colon26 were inoculated subcutaneousely into male BALB/C mice to establish the routine cancer cachexia model. The forty BALB/C mice were divided into four groups- normal control group （isovolumic normal saline）, model （isovolumic normal saline） group and luteolin high-dose and low-dose groups （100, 50 mg/kg）. Mice were killed, they were treated once a day for 7 d by ig administration. Net quality, dry weight, gastrocnemius muscle weight, epididymal fat weight, body fat mass and tumor weight in mice were detected by analytic balance. The serum triglyceride （TG）, esterification of fatty acid （NE- FA）, total cholesterol （TC） and glucose content were detected by automatic biochemical analyzer. The activities of calcium prote- ase and calcium activated protease in mice gastrocnemius muscle were determined by fuorometric method. Levels of serum TNF-o and IL-6 were determined by ELISA. RESULTS： Compared with normal control group, the model group showed a decrease in the net quality, dry weight, gastrocnemius muscle quality, epididymal fat mass and body fat mass, and a increase in the tumor weight; the levels of TG, NEFA,TC were increased, the glucose content decreased; the activities of calcium protease and calciunl activated protease in mice gastrocnemius muscle were enchanced; the serum levels of IL-6 were increased; the differences were sta- tistically signifcant （P〈0.01 or P〈0.05）, Compared with model group, in the luteolin high or low dose group, the net weight, dry weight, gastrocnemius muscle weight, epididymal fat weight were increased; TC, TG and NEFA serum levels were decreased, glucose levels were increased; the activities of calcium protease and calcium activated protease in mice gastrocnemius muscle were attenuated; the serum IL-6 level was decreased. The body fat mass in luteolin high dose group were increased; the differences were s
出处
《中国药房》
CAS
北大核心
2015年第7期882-885,共4页
China Pharmacy
基金
国家自然科学基金资助项目(No.81270508)
