摘要
目的:通过对不同活性检测方法的综合比较,筛选最适的胰高血糖素样肽1(GLP-1)或其类似物的体外活性检测方法,为GLP-1类似物的体外生物活性检测奠定基础。方法:以GLP-1作为阳性药物,用MTT方法检测其对RIN-m-5F、MIN6细胞增殖的影响;用ELISA方法检测其对INS-1、MIN6细胞胰岛素分泌量的影响;用ELISA方法检测其对BHK-GLP-1R细胞cAMP分泌水平的影响。通过对上述方法的综合比较,筛选出最适的活性检测方法。结果:GLP-1对细胞增殖的影响实验结果并不显著,对胰岛素分泌量影响的实验效果明显,对cAMP分泌水平的实验无明显效果。结论:ELISA检测GLP-1或其类似物对MIN6细胞胰岛素分泌量实验可用于GLP-1或其类似物的体外活性检测。
Objective： Through a comprehensive comparison of different kinds of active detection methods, in order to screening the most suitable bioactivity detection method for the detection of glucagon-like polypetide 1 （GLP-1） or GLP-1 analogue biological activity in vitro. Methods： GLP-1 protein was used as the positive drug to test its effect on the proliferation of RIN-m-5F and MIN6 cells by MTT method, on the amount of insulin secreted by INS-1 and MIN6 cells, and on the level of cAMP secreted by BHK-GLP-1R cells by ELISA method. After a comprehensive comparison of the above methods, screening out the optimal activity detection method. Results： The effect of GLP-1 on cell proliferation was not significant, on the insulin secretion achieved good results, on the level of cAMP secretion experiments no significant effect. Conclusion： The method for the detection of GLP-1 or GLP-1 analogue biological activity in vitro was established, in which ELISA was applied to test the ef- fect of GLP-1 or GLP-1 analogue on the amount of insulin secreted by MIN6 ceils.
出处
《生物技术通讯》
CAS
2015年第1期98-101,共4页
Letters in Biotechnology
基金
"十二五"重大新药创制国家科技重大专项"综合性新药研发技术大平台"(2012ZX09301003)
关键词
2型糖尿病
胰高血糖素样肽1
类似物
活性检测
type 2 diabetes mellitus
glucagon-like polypetide 1
analogue
activity detection
