摘要
目的探讨葡萄糖调节蛋白(GRP)78和GRP94蛋白在直肠腺癌的癌组织中的表达及其临床意义。方法收集直肠腺癌手术标本45例,常规石蜡包埋后切片,应用En Vision TM免疫组织化学法检测GRP78和GRP94蛋白在直肠腺癌的癌组织及相应癌旁组织中的表达水平。结果 1在45例直肠腺癌的癌组织中GRP78和GRP94蛋白表达阳性率明显高于其在对应的癌旁组织中的表达(GRP78:χ2=16.200,P<0.001;GRP94:χ2=14.221,P<0.001)。2 GRP78和GRP94蛋白在直肠腺癌的癌组织中的阳性表达均与病理分化类型、Dukes分期和淋巴结转移有关(P<0.05),尚未发现GRP78和GRP94蛋白在直肠腺癌的癌组织中的阳性表达与患者性别、年龄、肿瘤大小等临床特征有关(P>0.05)。3在24例GRP78蛋白表达阳性病例中,GRP94蛋白表达阳性者为18例,21例GRP78蛋白表达阴性病例中,GRP94蛋白表达阳性者为6例,两者在直肠腺癌中的表达呈正相关(rs=0.464,P<0.01)。结论 GRP78和GRP94蛋白在直肠腺癌的癌组织中呈高表达,并与其转移和侵袭有关,可作为判断直肠腺癌恶性程度的有用指标。
Objective To explore the expressions of glucose regulated protein（GRP） 78 and GRP94 in rectal adenocarcinoma and their clinical significances. Methods In 45 paraffin-embedded sections of rectal adenocarcinoma tissues and adjacent normal tissues, the expressions of GRP78 and GRP94 were examined by En Vision^TM. Results The positive rates of GRP78 and GRP94 protein expression in the rectal adenocarcinoma tissues were 53.33%（24/45） and 53.33%（24/45）, while those in the adjacent normal tissues were 13.33%（6/45） and 15.56%（7/45）, respectively. There was a statistical significance of the expression of GRP78 or GRP94 between the tumor tissues and the adjacent normal tissues（all P〈0.001）, and it was found that the positive expression rates were relevant to the extent of differentiation, Dukes stage, and lymph node metastasis of cancer（all P〈0.05）, but not to the patient＇s sex, age, and size of tumor（all P〉0.05）. And there was a statistical significance in Spearman method about the rate of positive expression between GRP78 and GRP94（rs=0.464,P〈0.01）. Conclusion GRP78 and GRP94 protein is highly expressed in rectal adenocarcinoma tissue, related to its metastasis and invasion, might be used as a useful indicator to judge the malignant degree.
出处
《中国普外基础与临床杂志》
CAS
2015年第2期204-207,共4页
Chinese Journal of Bases and Clinics In General Surgery
