摘要
背景:高糖环境下人牙周膜细胞会发生凋亡,其中bcl-2,bax是否启动?如何发挥作用?此方面尚未见有文献报道。目的:应用不同浓度葡萄糖影响人牙周膜细胞,观察细胞凋亡及bcl-2、bax基因表达的变化。方法:原代培养并鉴定人牙周膜细胞,取5-8代细胞用于实验。采用5.5 mmol/L葡萄糖(生理对照组)和25 mmol/L葡萄糖(高糖组)作用细胞24 h和48 h;以Hoechst 33258免疫荧光染色观察人牙周膜细胞凋亡;以Real-time PCR检测bcl-2、bax表达。结果与结论:25 mmol/L葡萄糖促进人牙周膜细胞凋亡,bcl-2、bax表达显著高于对照组(P<0.05);bcl-2/bax比值显著低于对照组(P<0.05)。结果说明高糖可增加人牙周膜细胞凋亡,Bcl-2家族发挥了重要作用。
BACKGROUND： High glucose can trigger the apoptosis of human periodontal ligament cells, in which, whether bcl-2 and bax are started？ How do they work？ There is still no relevant report. OBJECTIVE： To investigate the effect of high glucose on bcl-2 and bax mRNA expression as well as apoptosis in human periodontal ligament cells in vitro. METHODS： Human periodontal ligament cells were primarily cultured and identified. Then, cells at 5-8 passages were selected in this experiment. Cells were treated with 5.5 mmol/L （control group） and 25 mmol/L （high glucose group） glucose for 24 and 48 hours. Cell apoptosis was determined by Hoechst 33258 staining and the expression of bcl..2 and bax mRNA was detected by Real-time PCR. RESULTS AND CONCLUSION： High glucose （25 mmol/L） could induce the apoptosis of human Periodontal ligament cells and increase bcl-2 and bax significantly. The bcl-21bax ratio showed a more significant decline in the high glucose group compared with the control group （P 〈 0.05）. These findings indicate that high glucose can induce the apoptosis of human periodontal ligament cells, in which, Bcl-2 family plays an important role.
出处
《中国组织工程研究》
CAS
CSCD
2014年第51期8254-8260,共7页
Chinese Journal of Tissue Engineering Research
基金
the Project of Science and Technology Bureau of Qingdao,No.2012-1-3-1-(15)-nsh~~
