摘要
目的:研究外源性骨髓间充质干细胞(Bone marrow mesenchymal stem cells,BMSCs)在不同时间窗的移植对大鼠矽肺纤维化的疗效。方法:体外分离、培养雄性3周龄SD大鼠BMSCs传至3代备用。将50只SD雌性大鼠随机分为5组:对照组,矽肺模型组,早期治疗组,中期治疗组,晚期治疗组(n=10)。采用非暴露式气管插管一次性灌注二氧化硅(SiO2)粉尘混悬液法建立大鼠矽肺模型(50μg/ml),并分别于造模后1、14、28 d BMSCs干预治疗(1×106ml-1),各组大鼠分别于治疗后14 d处死取材。流式细胞仪鉴定BMSCs;HE染色观察肺组织形态学变化;免疫组织化学法及Western blot法检测MMP-9,Ⅰ型胶原和Ⅲ型胶原的定位和定量表达。结果:矽肺模型组较对照组大鼠有明显炎症细胞浸润、矽结节形成、胶原沉积等病理改变。BMSCs在矽肺早期(1 d)和中期(14 d)移植治疗较矽肺模型组病理改变有明显缓解(P<0.05);BMSCs在矽肺晚期(28 d)移植治疗无显著效果(P>0.05)。结论:BMSCs的移植在矽肺纤维化早中期对大鼠的病理进程起到延缓作用,晚期治疗效果不明显。
Objective： To explore the effect of bone marrow mesenchymal stem cell transplantation on silicosis fibrosis in different time windows in rats. Methods： BMSCs were isolated and cultured from male 3-week-old SD rats in vitro. Fifty healthy female SD rats were randomly divided into 5 groups：control group, silicosis model group, early treatment group, middle treatment group, late treatment group （n = 10）. The silicosis model was made by one-time infusion of silica dust suspension using the non-exposed tracheal intubation（50μg/ml）, and 1 d, 14 d,28 d of BMSCs were given for intervention therapy（ 1 × 10^6 ml^-1 ）. Rats in each group were sacrificed 14 days after treatment. The BMSCs identified by flow cytometry;the morphological changes of the lung tissues were observed by HE staining;the expression of MMP-9, collagen type I and collagen type Ⅲ were detected by immunoeytochemistry and Western blot analysis. Results： BMSCs in early silicosis （1 d） and the middle silicosis （14 d） compared to silicosis model group can significantly alleviate the pathological process of silicosis fibrosis （P〈0. 05 ）, BMSCs in late silicosis （28 d） treatment had no significant effect（ P〉0.05 ）. Conclusion： Exogenous BMSCs transplantation on rat silicosis early pathological processes play a role in delaying, late treatment effect is not obvious.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2015年第2期193-196,209,共5页
Chinese Journal of Immunology
基金
河北省科技支撑重点专项(09276191D)
河北省重点职业病防治技术研究(13277709D)
