摘要
目的:研究不同型号大孔吸附树脂对莱菔子水提液中萝卜苷的纯化效果,筛选适合萝卜苷纯化的最佳型号树脂。方法:以萝卜苷的纯化效率为指标,采用高效液相色谱法测定莱菔子水提液和洗脱液中萝卜苷的含量,选择最佳型号大孔树脂。结果:X-5型大孔吸附树脂对萝卜苷的纯化率为86.2%,表明X-5型树脂更适合莱菔子中萝卜苷的纯化。萝卜苷在X-5型树脂上的吸附平衡时间为4 h。结论:采用X-5型大孔吸附树脂纯化莱菔子中萝卜苷是可行的。
Objective：To study the purification effect of different types of macroporous resin on gluco-raphenin in water extract of Raphani Semen,so as to find the best macroporous resin for the purification of Glucoraphenin. Methods：With purification rate of glucoraphenin as indicator , the HPLC method was used to determine the content of glucoraphenins in water extract and eluant of raphani semen ,so as to select the better vesin. Results：The purification rate of glucoraphenin by X-5 resin was 86.2%,which showed that X-5 resin was suit to purify glucoraphenin in water extract of Raphani Semen. The adsorption equilibrium time of glucoraphenin in X-5 was 4 hours. Conclusion：It is feasible to apply X-5 resin to purity glucoraphenin.
出处
《山东中医药大学学报》
2015年第1期88-90,共3页
Journal of Shandong University of Traditional Chinese Medicine
基金
山东省自然科学基金项目(编号:ZR2014HQ005)
山东省高等学校科技计划项目(编号:J105LL05)
山东中医药大学青年骨干培养计划课题(编号:ZYDXY1332)
关键词
莱菔子
大孔吸附树脂
萝卜苷
Raphani Semen
Macroporous resin
glucoraphenin
