摘要
目的对体外培养的人大骨节病和骨关节炎软骨细胞添加不同剂量透明质酸,观察干预前后软骨细胞透明质酸合成酶2(HAS2)mRNA的变化。方法选取2006年8月—2008年7月陕西地方病研究所明确诊断大骨节病,并实施膝关节游离体摘除术后取出游离体及关节软骨6例为大骨节病组;西安市红十字会医院明确诊断骨关节炎,并行全膝关节置换术后的膝关节软骨6例为骨关节炎组;另选择同时期由于意外车祸等原因截肢或身亡者的新鲜膝关节软骨6例为对照组。对3组软骨细胞采用不同剂量的透明质酸进行干预,分为0μg/ml(H0)、100μg/ml(H100)、500μg/ml(H500),通过RT-PCR法观察透明质酸对3组软骨细胞HAS2 mRNA表达的影响。结果干预前3组HAS2 mRNA表达比较,差异有统计学意义(P<0.05);其中骨关节炎组较对照组降低(P<0.05)。干预后3组不同剂量间HAS2 mRNA表达比较,差异均无统计学意义(P>0.05)。结论退变的大骨节病和骨关节炎软骨细胞合成透明质酸能力下降;应用外源性透明质酸后,有增加软骨细胞自身透明质酸合成的趋势,为透明质酸关节腔注射治疗大骨节病及骨关节炎提供了理论基础。
Objective To investigate the effect of hyluronic acid（ HA） on the expression of HAS2＇s mRNA in chondrocytes of Kashin- beck disease（ KBD） and osteoarthritis（ OA） cultured in vitro. Methods A total of 6 patients who were diagnosed with KBD in Shanxi Provincial Institute for Endemic Disease from August 2006 to July 2008,were selected as KBD group,the loose bodies and articular cartilage of KBD patients were removed through surgery. A total of 6 patients who were diagnosed with OA in Xi＇an City Red Cross Hospital,were selected as OA group,the knee articular cartilage of OA patients were removed through total knee arthroplasty. At the same time,amputees and the dead（ 6 cases） due to accidents such as traffic accident,were selected as control group. Cells from 3 groups were treated with different doses of HA（ 0 μg /ml,100 μg /ml and 500μg/ml）,the effect of HA on the expression of HAS2＇s mRNA in 3 groups of chondrocytes was detected by RT- PCR. Results Before treatment,there were significant differences in the expression levels of HAS2＇s mRNA among 3 groups of chondrocytes（ P〈0. 05）. The HAS2＇s mRNA expression level in OA group was significantly lower than that in control group（ P〈0. 05）. After treatment,there was no significant difference in the expression levels of HAS2＇s mRNA among different dose groups（ P〉0. 05）.Conclusion The capacity of HA synthesis decreases in chondrocytes of KBD and OA. The exogenous HA can enhance chondrocytes＇ capacity of HA synthesis,which provides the theory foundation for treating KBD and OA with intraarticular injection of HA.
出处
《中国全科医学》
CAS
CSCD
北大核心
2014年第30期3575-3578,3584,共5页
Chinese General Practice
基金
国家自然科学基金重点项目(30630058)
科技部国际合作重点专项基金(2006DFA33610)
陕西省社发公关基金(2013SF2-10)
