摘要
目的探讨人类白细胞抗原B 27(HLA-B 27)检测对诊断强直性脊柱炎(AS)的临床意义。方法用流式细胞仪检测651例AS患者、1 085例非AS患者(包括腰腿背痛患者、风湿性关节炎患者、类风湿性关节炎患者)及200例健康体检者3组人群的HLA-B 27抗原。结果 AS患者组HLA-B 27阳性率为92.6%,非AS患者组HLA-B 27阳性率为9.6%,健康体检组HLA-B 27阳性率为4.5%,AS患者组HLA-B 27阳性率明显高于非AS患者组和健康体检组,AS患者组与非AS患者组、健康体检组差异有统计学意义(χ2=1 303.01,P<0.01)。男性HLA-B 27阳性率为47.4%,女性HLA-B 27阳性率为14.6%,男性HLA-B 27阳性率明显高于女性(χ2=193.17,P<0.01)。不同年龄组HLA-B 27阳性率差异有统计学意义,<30岁年龄组的阳性率明显高于其他年龄组。结论 HLA-B 27抗原在AS发病过程中起了重要作用,具有很强的相关性,故检测HLA-B 27可为AS的诊断和鉴别诊断提供重要依据。
Objective To investigate the clinical significance of human leukocyte antigen B 27（ HLA- B 27） detection in the diagnosis of ankylosing spondylitis（ AS）. Methods Flow cytometry was used to detect HLA- B 27 antigen in 651 cases of AS patients,1 085 non AS patients（ including patients,low back pain in patients with rheumatic arthritis,rheumatoid arthritis patients） and 200 cases of healthy people. Results The positive rate of AS in patients with HLA- B 27 was 92. 6%,the positive rate of HLA- B 27 in patients with non AS was 9. 6%,the positive rate of HLA- B 27 in healthy volunteers was 4. 5%,the positive rate of AS in group HLA- B 27 was significantly higher than that of non AS patients and healthy controls,the difference of healthy group of AS patients and in patients with non AS group（ χ^2= 1 303. 01,P〈0. 01）. Male HLA- B 27 positive rate was 47. 4%,the positive rate of HLA- B 27 was 14. 6% in women,the positive rate of HLA- B 27 in male was higher than female,the difference was statistically significant（ χ^2= 193. 17,P〈0. 01）. There was statistical significance in the positive rate difference of HLA- B 27 in different age groups,the positive rate less than 30 years old group was significantly higher than in other age groups. Conclusion The HLA- B 27 antigen plays an important role in the pathogenesis of AS,has a strong correlation,so the detection of HLA- B 27 can provide an important basis for the diagnosis and differential diagnosis of AS.
出处
《中国卫生检验杂志》
CAS
2015年第1期65-66,共2页
Chinese Journal of Health Laboratory Technology
