摘要
目的：比较实时 PCR 法和 PCR-反向点杂交法（PCR-RDB）检测 HPV 的一致性。方法收集109份女性生殖道样本，利用实时 PCR 法和 PCR-RDB 法分别检测 HPV 感染和基因型分布情况，不一致样本采用 PCR-悬浮芯片杂交法复检。结果83.5％（91／109）的样本两种方法结果一致（kappa＝0.671），18例不一致样本 PCR-悬浮芯片杂交法复检显示7例与实时 PCR相符，11例与 PCR-RDB 相符；高、低病毒载量组间 PCR-RDB 法的检测结果差异无统计学意义（χ2＝1.476，P ＝0.224）。结论实时 PCR 和 PCR-RDB 两法用于 HPV 检测一致性一般；HPV 病毒载量在103～108范围内时 PCR-RDB 法的阳性率较稳定。
Objective To compare real time PCR with PCR-reverse dot blot hybridization （PCR-RDB）for detecting human pap-illomavirus （HPV）infection in women.Methods A total of 109 genital specimens from women were collected in the study.All specimens were tested HPV by using real time PCR and PCR-RDB,discrepant samples were tested again by PCR-xMAP.Results The concordant rate was 83.5%（91/109）between real time PCR and PCR-RDB （kappa=0.671）,the other 18 discrepant samples were retested by PCR-xMAP,7 of those were identical with real time PCR and 11 with PCR-RDB.No differences of PCR-RDB pos-itive rates were found between the high and low viral load groups （χ2 =1.476,P =0.224）.Conclusion It demonstrated moderate consistency between real time PCR and PCR-RDB.The HPV positive rates of PCR-RDB were stable when the viral loads were 103-108 .
出处
《国际检验医学杂志》
CAS
2014年第24期3373-3374,3376,共3页
International Journal of Laboratory Medicine
基金
宝安区科技计划项目(2009355)
关键词
人乳头状瘤病毒
实时PCR法
PCR-反向点杂交法
基因型
human papillomavirus
real time polymerase chain reaction
polymerase chain reaction-reverse dot blot hybrid-ization
genotype
