摘要
为了研究外源SO2对紫苏种子萌发及其幼苗生理生化的影响,用不同浓度的SO2(0,0.5,1,1.5,2,2.5 mmol/L)处理紫苏种子,使其在适当的条件下萌发,测定其种子的发芽率、发芽势及发芽指数,以及幼苗叶绿素、丙二醛(MDA)、过氧化物酶(POD)、多酚氧化酶(PPO)及可溶性糖含量。结果表明,随着SO2浓度的升高,发芽率、发芽势、发芽指数、POD活性和可溶性糖含量都呈先上升后下降的趋势,在1.5 mmol/L时达到最大;叶绿素含量随着SO2浓度的升高,呈逐渐上升的趋势;MDA含量、PPO活性则随着SO2浓度的升高,呈先下降后上升的趋势,在1.5 mmol/L时到达最低。实验表明用1.5 mmol/L浓度的外源SO2处理紫苏种子有利于其萌发和生长,可以提高幼苗的抗氧化胁迫能力。
The study aims to investigate the implantation of exogenous SO2 on Perilla frutescens seeds germination and seedling membrane physiology and biochemical. The research used various concentrations of SO2（0, 0.5, 1, 1.5, 2, 2.5 mmol/L） to treat Perilla frutescens seeds to germinate under appropriate conditions,and determine its seeds germination rate, germination potential and germination index, as well as chlorophyll,MDA（malondia idehyde）, POD（peroxidase）, PPO（polyphenoloxidase）, soluble sugar content of seedlings. The results showed that with the SO2 concentration increased, the germination rate, germination potential,germination index, POD activity and soluble sugar content were first increased and then decreased, the maximum reached 1.5 mmol/L. Chlorophyll content generally showed a gradual upward trend with the increase of SO2 concentration. MDA content, PPO activity along with the increase of SO2 concentration, a generally upward trend after the first fall, were lowest in 1.5 mmol/L. What the figure showed that in the SO2 concentration of 1.5 mmol/L was most favorable for Perilla frutescens seeds germination and growth, and could improve seeds antioxidant stress capacity.
出处
《中国农学通报》
CSCD
2014年第31期151-155,共5页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金项目"紫苏ALA代谢积累关键基因的克隆
表达分析及功能研究"(31171588)
关键词
SO2
紫苏
发芽率
膜氧化
抗氧化酶
SO2
Perilla frutescens
germination rate
membrane oxidation
anti-oxidizing enzyme
