摘要
谷氨酰胺合成酶（GS，EC6.3.1.2）是氮素代谢途径中的关键酶，对植物的生长和发育至关重要。采用多种生物信息学软件对6个玉米G5基因的可变剪接现象、核苷酸序列、编码蛋白序列、磷酸化位点、基因组结构、同源进化关系以及启动子顺式作用元件进行系统的生物信息学分析。结果表明，5个玉米GS基因存在可变剪接现象，编码蛋白的氨基酸数目为356-423个；6个玉米GS蛋白存在19-38个的磷酸化位点，各成员基因组序列上含有10-15个外显子；玉米G5基因可划分为GS2、GS1-1、GS1-2和GS1-3等4个亚组；6个基因定位到1、4、5、9和10号共5条染色体上，6个成员的启动子区域含有MYB结合位点、水杨酸响应元件和茉莉酸甲酯响应元件等重要的调控元件。
Glutamine synthetase is one of the most important enzyme in nitrogen metabolism, and plays a crucial role in the growth and development of the plant. By bioinformatics analysis, the alternative splicing phenomenon, nucleotide sequence, encoded protein, phosphorylation sites, genomic structure, evolutionary relationship and cis-trans elements in the promoter of six GS genes in maize were analyzed. The results showed that 5 GS genes were found to be subjected to alternative splicing, the encoded proteins contain 356-423 amino acids, and all of them had phosphorylation sites on the protein sequences. Genomic structure analysis consequence indicated that the genome sequences of the 6 GS genes formed by 10-15 exons and introns. The result of phylogenetic analysis revealed that the cytosolic GS genes were organized in three functionally and phylogenetically conserved subfamilies. Chromo-some mapping analysis showed that they could be located on 1, 4, 5, 9 and 10 chromosomes. Cis-trans elements prediction results demonstrated that all promoters of them had MYB binding sites, salicylic acid responsive elements and MeJA responsive elements etc. The bioinformatics analysis results will provide a basis for further charac-terization of biological function and regulation mechanism of GS genes in maize.
出处
《玉米科学》
CAS
CSCD
北大核心
2015年第1期51-57,共7页
Journal of Maize Sciences
基金
山西农业大学博士启动基金(2013YJ07)
山西农业大学科技创新基金(2014020)
