摘要
目的：建立测定贵州产香薷和野草香药材中总黄酮的含量测定方法。方法：采用紫外-可见分光光度法,以芦丁为对照品,在482 nm波长处对两种香薷属植物中的总黄酮进行含量测定。结果：芦丁的质量浓度在0.02～0.1 mg/ml范围内与吸光度值呈良好的线性关系;精密度、稳定性、重复性试验的RSD〈2%;平均加样回收率为97.55%,RSD为0.66%（n=6）。香薷和野草香的花、茎叶及根中总黄酮的含量分别为0.704、0.438、0.247 mg/g和0.901、0.476、0.352 mg/g。结论：该方法操作简便、准确,重复性和稳定性良好,适用于香薷属植物总黄酮的含量测定。
OBJECTIVE： To establish a method for the determination of total flavonoids content in Elsholtzia ciliata and Elsholtzia cypriani produced in Guizhou. METHODS：The total flavonoids contents in two Elsholtzia plants were determined by UV at 482 nm with the reference of rutinum. RESULTS ： The concentration of rutinum had a good linear relationship with absorbance in the range of 0.02-0.1 mg/ml. The RSDs of precision, stability and repeatability test were less than 2% ; the average sample recov- ery were 97.55%, RSD were 0.66% （n=6）.The contents of total flavonoids in the flowers, stem leaf and root of E. ciliata and E. cypriani were 0.704, 0.438,0.247 mg/g and 0.901, 0.476, 0.352 mg/g. CONCLUSIONS： This method is simple and accurate and has good repeatability and stability. It is applicable for the determination of total flavonoids contents in Elsholtzia plants.
出处
《中国药房》
CAS
北大核心
2015年第6期805-807,共3页
China Pharmacy
基金
贵州省科技计划课题(No.黔科合SY字〔2011〕3086)
关键词
香薷
野草香
总黄酮
提取
紫外-可见分光光度法
含量测定
Elsholtzia ciliata
Elsholtzia cypriani
Total flavonoids
Extract
UV
Content determination
