摘要
A multi-layered composite scaffolds consisting of poly （ L- ne） （ P （LLA-CL） ）, collagen （COL） and chitosan （CS） were fabricated by a bi-directional electrospinnlng method. Synthetic P （LLA-CL） was used as the middle layer to enhance the strength, while natural COL/CS blending （9： 1, v/v） was used as the bioactive surfaces （inner and outer layers ） to improve the biocompatibility. Each three transitional layers were set between inner/outer layer and middle layer for delamination resistance. Scanning electron microscopy （SEM） was used to observe the fiber morphology. The Fourier transform infrared attenuated total reflectance spectroscopy （FTIR-ATR） spectra, X- ray diffraction （XRD） and thermogravimetry （TG） tests were used to analyze the physical properties of the scaffolds. The results showed that the modified clectrospinning method bad no negative effect on the components, crystal structure and thermostability of the scaffolds, but could effectively combine the mechanical property of synthetic material and biocompatibility of natural materials. Such method could be applied to the fabrication of composite scaffolds for vascular, skin. and nerve tissue engineering.
A multi-layered composite scaffolds consisting of poly( Llactide-co-ε-caprolactone)( P( LLA-CL)),collagen( COL) and chitosan( CS) were fabricated by a bi-directional electrospinning method.Synthetic P( LLA-CL) was used as the middle layer to enhance the strength,while natural COL / CS blending( 9∶ 1,v / v)was used as the bioactive surfaces( inner and outer layers) to improve the biocompatibility.Each three transitional layers were set between inner / outer layer and middle layer for delamination resistance.Scanning electron microscopy( SEM) was used to observe the fiber morphology.The Fourier transform infrared attenuated total reflectance spectroscopy( FTIR-ATR) spectra,Xray diffraction( XRD) and thermogravimetry( TG) tests were used to analyze the physical properties of the scaffolds.The results showed that the modified electrospinning method had no negative effect on the components,crystal structure and thermostability of the scaffolds,but could effectively combine the mechanical property of synthetic material and biocompatibility of natural materials.Such method could be applied to the fabrication of composite scaffolds for vascular,skin,and nerve tissue engineering.
基金
"111 Project" Biomedical Textile Materials Science and Technology,China，National Natural Science Foundations of China，Science and Technology Commission of Shanghai Municipality,China，Ph.D.Programs Foundation of Ministry of Education of China
