摘要
目的:探讨CD8+CD28-抑制性T细胞在乳腺癌手术患者外周血中的含量及其TGF-β1和IL-10的表达及临床意义。方法:以健康人及良性乳腺增生患者为对照,选取60例乳腺癌患者,其中手术患者43例,采用流式细胞术检测手术前后静脉血CD8+CD28-抑制性T细胞及其表达的细胞因子转化生长因子β1(TGF-β1)及白介素10(IL-10)的百分含量;并将三者与肿瘤分期进行相关性分析。结果:乳腺癌患者与健康人及良性乳腺增生患者比较,CD8+CD28-抑制性T细胞、TGF-β1及IL-10均明显升高(P<0.05),而良性乳腺增生患者与健康人无统计学差异(P>0.05)。手术后,乳腺癌患者CD8+CD28-抑制性T细胞、TGF-β1及IL-10均较术前明显下降(P<0.05)。相关性分析显示CD8+CD28-抑制性T细胞、TGF-β1及IL-10均明显升高,且与肿瘤分期呈显著正相关(r>0.7,P<0.05)。结论:CD8+CD28-抑制性T细胞、TGF-β1及IL-10均升高可能是乳腺癌重要的发病机制之一,且与肿瘤分期密切相关,具有重要临床意义。
Objective：To study the expression and clinical significance of CD8 ＋CD28 - suppresser T cells for patients with breast cancer undergoing radical mastectomy.Methods：Sixty cases with breast cancer,including 43 individuals undergoing surgery,were collected as the observation group.Fifty patients with breast hyperplasia and 50 healthy persons were enrolled as the control group.The levels of blood CD8 ＋ CD28 - suppresser T cells,growth factors and cytokines 1 （TGF-beta 1 ）and interleukin 10 （IL-10 ）were detected via flow cytometry for the upper three groups.The correlation between CD8＋CD28-T cells,TGF-beta 1,IL-10 and the tumor stage were analyzed.Results：Compared with healthy people and patients with breast hyperplasia,CD8 ＋ CD28 - T cells,TGF-beta 1 and IL-10 were significantly increased in patients with breast cancer（P 0.05）.CD8＋CD28-T cells,TGF-beta 1 and IL-10 in patients with breast cancer were significantly decreased when compared with pre-operation（P 0.7,P 〈0.05）.Conclusion：The increase of CD8＋CD28-T cells,TGF-beta 1 and IL-10 could likely to be one of the important pathogenesis of breast cancer.They are closely related to the tumor stages and therefore the results imply important clinical significance.
出处
《长治医学院学报》
2014年第6期410-413,共4页
Journal of Changzhi Medical College
