摘要
目的:探讨尼莫地平对缺血再灌注大鼠脑组织细胞凋亡和细胞色素C蛋白(cytochrome C,Cytc)表达的影响。方法:将大鼠随机分为假手术组、模型组、尼莫地平组,每组27只。采用线栓法栓塞大脑中动脉3h制作大鼠脑缺血再灌注模型;分别于6h、12h、24h用TUNEL法检测大脑皮层细胞凋亡、Western blot检测大脑皮层Cytc蛋白的表达。结果:TUNEL法和Western blot结果显示:(1)尼莫地平组各时间点神经细胞凋亡较模型组明显减少(P<0.01);(2)模型组Cytc蛋白各时间点表达较假手术组有显著性差异(P<0.01、P<0.05);(3)尼莫地平组各时间点较模型组有显著性差异(P<0.01)。结论:尼莫地平对脑缺血再灌注损伤保护机制与抑制Cytc蛋白表达有关。
Objective：To investigate the effects of nimodipine on cell apoptosis and the expression of cytochrome c in the brain tissue after cerebral arterial thrombosis in rats.Methods：Rats were randomly divided into three groups：the sham-operative group,the model group and the nimodipine group（n = 27 in each group）.The model of middle cerebral artery occlusion for 3 h was induced by suture method.The apoptosis of perifocal cortex was detected by TUNEL method and the expression of cytochrome c was analyzed by Western blot at the end of 6 h,12 h and 24 h.Results The results of TUNEL and Western blot showed：（1）Compared with the model group,the apoptosis decreased obviously in the nimodipine group （P 〈0.01）;（2）Compared with the sham group,the expression of cytochrome c in the perifocal cortex of the model group was significantly different（P 〈0.01）;（3）the expression of cytochrome C was reduced significantly in the nimodipine group at every time compared with the model group（P 〈0.01）.Conclusion：Nimodipine can protect the brain cells from damage after cerebral arterial thrombosis injury by adjusting the expression of cytochrome C.
出处
《长治医学院学报》
2014年第6期401-404,共4页
Journal of Changzhi Medical College
基金
江苏省"333高层次人才培养工程"科研项目资助(BRA2012050)
江苏大学医学临床科技发展基金(JLY2010053)
常州市卫生局资助项目(WZ201043)
关键词
尼莫地平
脑缺血再灌注
凋亡
细胞色素
C
大鼠
Nimodipine
Cerebral ischemia reperfusion
Apoptosis
Cytochrome C
rat
