摘要
目的探讨β-内酰胺类抗菌药物对婴儿肠道菌群的影响。方法选取0~1岁婴儿,应用16S rRNA荧光定量PCR技术,分别测定β-内酰胺类抗菌药物使用前,使用第3d、5天时,及治愈后第7天粪便中双歧杆菌、乳酸杆菌、肠球菌、大肠杆菌的水平。结果使用过β-内酰胺类抗菌药物者粪便中双歧杆菌、乳酸杆菌、大肠杆菌和肠球菌的数量与未使用者比较,差异无统计学意义（P〉0.05）。粪便双歧杆菌、乳酸杆菌含量随治疗时间的延长而增加,差异有统计学意义（P〈0.05）。未使用抗菌药物的患儿肠道双歧杆菌和乳酸杆菌的恢复明显快于使用过抗菌药物的患儿,差异有统计学意义（P〈0.05）。结论β-内酰胺类抗菌药物对婴儿肠道菌群有普遍的杀灭作用,对婴儿肠道菌群改变的影响是轻微的。β-内酰胺类抗菌药物的使用会延迟患儿肠道微生态的恢复,但若合理使用对疾病的治疗有积极作用。
Objective To investigate the influence ofβ-lactam antimicrobial drugs on infants′intestinal flora.Methods Infants from 0to 1years old were enrolled in the study,who′s feces samples were tested for Bacillus bifidus,lactobacillus,enterococcus and E.coli by using 16 SrRNA quantitative PCR technique before using antibiotics,on the third and fifth day duringβ-lactam antibiotics treatment and the seventh day after treatment.Results The numbers of Bacillus bifidus,lactobacillus,E.coli and enterococci detected in usingβ-lactam antibiotics group were not statistically different from those in not using antibiotics group（P〉0.05）.The numbers of Bacillus bifidus and lactobacillus increased with the treatment process,the differencies was statistically significant（P〈0.05）.The recovery of the numbers of intestinal Bacillus bifidus and lactobacillus in not using antibiotics group was significantly faster than using antibiotics group,the difference was statistically significant（P〈0.05）.Conclusion β-lactam antibiotics have general killing effect on the intestinal flora in infants,which could recover to normal.The recovery of intestinal flora could be delayed if antibiotics are used,however,reasonable antibiotics treatment would be very helpful in the treatment of primary diseases.
出处
《国际检验医学杂志》
CAS
2015年第3期357-359,共3页
International Journal of Laboratory Medicine
