摘要
目的探讨上皮性卵巢癌组织中WT1、Bcl-2、Ki-67蛋白的表达及其相关性。方法采用免疫组化方法检测62例上皮性卵巢癌组织及10例正常卵巢组织中WT1、Bcl-2、Ki-67蛋白的表达情况。结果⑴10例正常卵巢组织中WT1、Ki-67蛋白均无阳性表达、Bcl-2蛋白1例阳性表达;62例上皮性卵巢癌中WT1、Bcl-2、Ki-67阳性蛋白表达率分别为80.64%(50/62)、53.23%(33/62)、82.26%(51/62)。⑵上皮性卵巢癌组织中WT1、Bcl-2、Ki-67蛋白的表达率高于正常组织(P<0.05);WT1、Ki-67蛋白阳性表达与组织学分级和临床病理分期相关(P<0.05),Bcl-2蛋白阳性表达与组织学分级和临床病理分期不相关(P>0.05)。⑶WT1蛋白表达与Bcl-2蛋白的表达正相关(r=0.463,P<0.05),WT1蛋白与Ki-67蛋白,Ki-67蛋白与Bcl-2蛋白表达之间无相关性(r=0.012、r=0.120、P>0.05)。结论 WT1、Bcl-2、Ki-67蛋白表达在上皮性卵巢癌的发生发展过程中起重要作用,WT1可能通过上调Bcl-2抑制细胞凋亡促使卵巢癌的发生。
Objective To assess the expression of WT1,Bcl-2,Ki-67 in epithelial ovarian carcinoma and to analyze the correlation of the expression level of WT1,Bcl-2 and Ki-67,protein.Methods Consecutive sections from 62 cases of ovarian cancer and 10 normal ovarian tissues were assessed immunohistochemically for expression of WT1,Bcl-2 and Ki-67 protein.Results ⑴WT1 and ki-67 proteins were not detectable in normal ovarian tissues.Positive expression of Bcl-2 protein was observed in 1（10%） of 10 normal ovarian tissues.Positive expressions of WT1,Bcl-2 and Ki-67 proteins were 80.64%（50/62）,53.23%（33/62）and 82.26%（51/62） in ovarian cancer respectively.⑵The expression of WT1,Bcl-2 and Ki-67 protein in ovarian cancer was significantly higher than that in normal ovarian tissues（P〈0.05）.The expressions of WT1,Bcl-2 and ki-67 protein were respectively related to histological grade and FIGO stage（P〈0.05）.The expressions of Bl-2 protein was not related to histological grade and FIGO stage（P〈0.05）.⑶A significantly positive relationship was observed between WT1 and Bcl-2 protein expression in primary epithelial ovarian cancer（r=0.463,P〉0.05）.There was no correlation beween the expressions of WT1 and ki-67,Bcl-2 and ki-67 proteins in epithelial ovarian cancer（r=0.012、r=0.120,P〈0.05）.Conclusion These results suggest that WT1 and ki-67 may be involved in the occurrence and development of ovarian cancer.WT1 by upregulating Bcl-2 inhibits apoptosis promote ovarian cancer.
出处
《江西医药》
CAS
2015年第1期16-19,共4页
Jiangxi Medical Journal
基金
江西省上饶市科技计划(20142CZD04)
