摘要
研究了3种大孔吸附树脂D3520、DS-8和DM-301对内生真菌Berkleasmium sp.Dzf12中螺二萘类化合物Palmarumycins C12和C13积累的影响。3种树脂均显著促进Palmarumycin C12产量的提高,仅树脂DM-301同时有利于Palmarumycins C12和C13产量的提高,总产量中81.3%~95.8%的Palmarumycin C12被大孔树脂吸附。当树脂DM-301以浓度4.17%(g/m L)于第9 d添加到内生真菌Dzf12培养液、共培养112 h后,Palmarumycins C12和C13的产量分别达到122.72 mg/L和55.99 mg/L,分别为对照(2.19 mg/L和29.86 mg/L)的56.04倍和1.88倍。结果表明,在内生真菌Dzf12液体发酵培养中添加大孔吸附树脂能有效提高螺二萘类化合物Palmarumycins C12和C13的产量。
The effects of three macroperous adsorDtlon resins D3520, DS-8 and DM-301 on the production of palmarumy- clns Cx2 and CI3 in liquid culture of endophytic fungus Berkleasmium sp. Dzfl2 were investigated in this study. All the tested resins enhanced the production d palmarumycin Ct2. Only resin DM-301 enhanced the production of both palma- rumycins Cl2 and Cl3.81.3% to 95.8% of palmarumycin CI2 was distributed in the resins. When resin DM-301 was ap- plied to the medium at 4. 17% （g/mL） on day 9,and then harvested after 112 h incubation,the maximal yields d pal- mannnycins C12 and C13 were obtained as 122.72 mg/L and 55.99 mg/L,which were 56.04-fold and 1.88-fold in corn- parlson with tho6e （2.19 mg/L and 29.86 rag/L） d the control,respectlvely. It can be an effective strategy for produ- cing palmarumycins C12 and C13 in future large-scale culture of Berlde^mium sp. Dzf12 by adding macroperous resins.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2015年第2期205-209,240,共6页
Natural Product Research and Development
基金
国家高技术研究发展计划(2011AA10A202)
国家自然科学基金(31071710)
