摘要
目的研究蜂胶总黄酮(TFP)对自发性糖尿病大鼠糖脂代谢的影响。方法将50只自发性糖尿病大鼠按血糖值随机分为对照组、消渴丸组(2 000 mg·kg-1)、蜂胶总黄酮高剂量组(240 mg·kg-1)、中剂量组(120 mg·kg-1)、低剂量组(60mg·kg-1)。各组大鼠每天灌胃给药1次,连续8周,对照组灌胃同体积羧甲基纤维素钠溶液。末次给药结束后,禁食12 h,乙醚麻醉,腹主动脉取血,检测自发性糖尿病大鼠血清中血糖(Glu)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、糖化血红蛋白(GHb)、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)、胰岛素(INS)、C肽(C-P)、肿瘤坏死因子(TNF-α)、游离脂肪酸(FFA)、一氧化氮(NO)及肝糖原水平。结果与对照组比较,蜂胶总黄酮高、中、低剂量组血糖、甘油三酯、总胆固醇、低密度脂蛋白胆固醇、丙二醛及肿瘤坏死因子-α水平明显降低(P<0.05或P<0.01或P<0.001),高密度脂蛋白胆固醇、超氧化物歧化酶、胰岛素、C肽及肝糖原含量明显升高(P<0.05或P<0.01或P<0.001);蜂胶总黄酮高、中剂量组糖化血红蛋白及游离脂肪酸水平明显降低(P<0.05或P<0.01或P<0.001),谷胱甘肽及一氧化氮含量明显升高(P<0.05或P<0.01),低剂量组则无显著性差异(P>0.05)。消渴丸组超氧化物歧化酶、肝糖原、一氧化氮及C肽明显升高(P<0.05或P<0.01),血糖、丙二醛、甘油三酯及糖化血红蛋白水平明显降低(P<0.05或P<0.01或P<0.001),对其他指标则无明显影响(P>0.05)。结论蜂胶总黄酮可明显降低自发性糖尿病大鼠血糖水平,明显改善自发性糖尿病大鼠糖脂代谢及抑制胰岛素抵抗。
OBJECTIVE To study on glucolipid metabolism effect of the total flavones of propolis （TFP） in Goto-Kakisaki rats （ GK Rats） . METHODS Fifty GK rats were randomly divided into 5 groups by blood glucose, the model group, positive drug group （2 000mg·kg^-1） and the high（240 mg·kg^-1） dose of the TFP group, the medium（ 120 mg·kg^-1） doses of the TFP group, low （60 mg·kg^-1 ） doses of the TFP group. The GK rats in TFP groups were given with TFP by intragastric administration, continuously treated for 8 weeks, the control group were given equal sodium carboxymethylcellulose （CMC-Na） and positive drug group were given （2 000 mg·kg^-1） dose of Xiaoke pills by intragastric administration. The glucose （ Glu）, total cholesterol （ TC ）, triglyceride （ TG）, high density lipoprotein（ HDL-C）, low density lipoprotein（ LDL-C）, glycated hemoglobin（ GHb）, superoxide dismutase （SOD）, malondialdehyde （MDA） , glutathione （GSH） , serum insulin （INS） , C-peptide （C-P） , tumor necrosis factor-α （TNF-α） free fatty acid （ FFA）, nitric oxide （ NO）, and hepatic glycogen were measured at the end of the experiment. RESULTS Compared with the control group, the levels of GLU, TG, TC, LDL-C, GHb, MDA and TNF-α were decreased significantly （ P 〈 0. 05 or P 〈 0. 01 or P 〈 0. 001 ）, the levels of HDL-C,SOD, INS, hepatic glycogen and C-P were increased significantly in the high-dose group, medium-dose group and low-dose group（P 〈0. 05 or P 〈0. 01 or P 〈0. 001 ）. The levels of GHb and FFA were decreased significantly（P 〈0. 05 or P 〈0. 01 or P 〈0. 001 ） , the levels of GSH and NO were increased significantly（P 〈0. 05 or P 〈0. 01 ） in the high-dose group and medium-dose group, the other indexes had no significant difference （P 〉 0. 05 ） in the low-dose group. The levels of SOD, hepatic glycogen, NO and C-P were increased significantly（P 〈0. 05 or P 〈0.01 ）, the levels of GLU, MDA, TG and GHb were decreased s
出处
《中国药学杂志》
CAS
CSCD
北大核心
2015年第3期217-220,共4页
Chinese Pharmaceutical Journal
基金
国家"十一五"重大新药创制(2009ZX09103-418)
吉林省科技支撑计划(20110946)
关键词
蜂胶总黄酮
糖尿病
自发性糖尿病大鼠
糖脂代谢
胰岛素抵抗
total flavones of propolis
diabetes mellitus
GK rats
glucolipid metabolism
insulin resistance
