摘要
基于表达谱数据分析,根据公共数据库中的序列信息设计引物,通过RT-PCR扩增及克隆,从耐冷的东乡野生稻(Dongxiang Oryza rufipogon Griff.)中获得了在冷胁迫条件下高表达的热激转录因子BGIOSGA004402-DX的全长c DNA,与冷敏感水稻品种93-11的对应位点序列进行氨基酸同源比较分析,结果表明:该基因在2个不同材料中长度一样,都为249个氨基酸,具有99%的序列一致性,但存在2个氨基酸的差异,一个是第127号氨基酸,另一个是第155号氨基酸。
The full length c DNA of high expression of heat shock transcription factor BGIOSGA004402-DX was obtained and cloned from cold-tolerant Dongxiang wild rice（ Oryza rufipogon Griff） based on analysis of expression profile data by reverse transcription polymerase chain reaction（ RT-PCR） with the primers designed on the basis of sequence information in public database. The comparison results of amino acid sequence with corresponding locus of cold-sensitive rice variety 93-11 indicated that the length of the gene had the same size of 249 amino acids in two different materials,with 99% of the sequence of consistency. However,the difference of two amino acids in the gene was detected in two different varieties. One was No. 127,and the other was No. 155.
出处
《杂交水稻》
CSCD
北大核心
2015年第1期64-67,73,共5页
Hybrid Rice
基金
"十一五"国家科技支撑计划(2009AAQ04002
2011AA10A101)
国家转基因作物新品种培育重大专项(2011ZX08001-001
2011ZX08001-004)
杂交水稻国家重点实验室(湖南杂交水稻研究中心)开放课题基金(2014KF04)
国家自然科学基金(31401456)
湖南工业大学自然科学研究计划项目(2014HZX02)
