摘要
【目的】了解上海市动物源食品中山夫登堡沙门菌Salmonella Senftenberg的流行情况、耐药性和分子分型情况.【方法】于2008—2012年在上海分离鉴定得到15株动物食品源山夫登堡沙门菌,通过琼脂稀释法进行最小抑菌浓度测定,并应用脉冲场凝胶电泳技术(PFGE)对菌株进行分子分型研究.【结果和结论】15株山夫登堡沙门菌有7株对磺胺异恶唑具有耐药性(46.7%),除1株对链霉素耐药外(6.67%),对其他抗生素均较为敏感.PFGE分型分为10个基因型.部分不同来源的基因型(X3,X4,X5)具有高度的相似性(88.2%),表明这些不同来源的菌株可能存在流行相关性.
【Objective】To investigate the antimicrobial resistance and molecular types of Salmonella Senftenberg isolates from food of animal origins in Shanghai. 【Method】A total of 15 Salmonella Senftenberg isolates were collected from 2008 to 2012 in Shanghai. Agar dilution method and pulse field gel electrophoresis（ PFGE） were applied to the determination of minimum inhibitory concentrations（ MICs） and molecular typing,respectively. 【Result and conclusion】The 15 isolates exhibited resistance most often to sulfisoxazole（ 46. 7%） and they were fully sensitive to the remaining antibiotics expect for one which was resistant to streptomycin（ 6. 67%）. PFGE analyses of 15 Salmonella Senftenberg isolates resulted in 10 unique patterns,among which three（ X3,X4,X5） were grouped together at a high similarity index of 88. 2%,su-ggesting that these isolates of different origins may be relevant in the epidemiology.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2015年第1期113-116,共4页
Journal of South China Agricultural University
基金
公益性行业(农业)科研专项(201303044)
关键词
山夫登堡沙门菌
动物源食品
最小抑菌浓度
抗菌素耐药性
脉冲场凝胶电泳
Salmonella Senftenberg
food of animal origins
minimum inhibitory concentration
antimicrobial resistance
pulse field gel electrophoresis
