摘要
It is well known that excessive long-term alcohol consumption is harmful, especially in pregnant women. In the present study, the Kunming white mouse was used as an animal model and indirect immunofluorescence was performed to analyze the toxic effects of alcohol on early pre-implantation embryos. H3K9 acetylation immunofluorescence could not be detected in MII oocytes. H3K9 acetylation levels in the treatment group were higher than in the control group during the morula stage, and contrary to results during the blastocyst stage. Other stages showed no obvious differences for in vivo embryos. For in vitro embryos, almost no difference was found between the two experimental groups across all stages, and both groups showed increasing H3K9 acetylation levels（except at the 2-cell stage）. This study shows that H3K9 acetylation levels in early pre-implantation embryos are notably impacted by excessive alcohol ingestion by females. These data are the first step in understanding the epigenetic mechanism of alcohol toxicity in early preimplantation mouse embryos.
It is well known that excessive long-term alcohol consumption is harmful, especially in pregnant women. In the present study, the Kunming white mouse was used as an animal model and indirect immunofluorescence was performed to analyze the toxic effects of alcohol on early pre-implantation embryos. H3K9 acetylation immunofluorescence could not be detected in MII oocytes. H3K9 acetylation levels in the treatment group were higher than in the control group during the morula stage, and contrary to results during the blastocyst stage. Other stages showed no obvious differences for in vivo embryos. For in vitro embryos, almost no difference was found between the two experimental groups across all stages, and both groups showed increasing H3K9 acetylation levels（except at the 2-cell stage）. This study shows that H3K9 acetylation levels in early pre-implantation embryos are notably impacted by excessive alcohol ingestion by females. These data are the first step in understanding the epigenetic mechanism of alcohol toxicity in early preimplantation mouse embryos.
基金
This study was supported by the National Natural Science Foundation of China （31372273, 31201789）
Academic Renovation Research Project of Anhui University （yqh100125）
Natural Science Foundation of the Education Bureau of Anhui Province （KJ2013A202）
Major project of discipline construction in Anhui province （[2014]No.28）
and Natural Science Foundation of Anhui Province （1408085MC44, 1408085QC65）ACKNOWLEDGEMENTS I would like to express my appreciation to Prof. Rong WANG, Dr. Ji-Chang HUANG and staff and students at our lab for generous help with this study.
