摘要
以浙江省6个异育银鲫规模化养殖场为监控点,于2013年6—7月与9—10月分别对每个养殖场进行采样与检测,所采样品均为表观健康个体。通过细菌分离、鉴定及毒力基因测定,对其肝、脾、肾携带细菌进行多样性分析。12个批次共检出7种细菌,均为人、兽、鱼共患病原菌。气单胞菌的检出率占据首位,气溶素、细胞毒性肠毒素、不耐热细胞紧张性肠毒素、耐热性细胞紧张性肠毒素为其主要毒力基因型,提示其可能具有致病性。鲁氏不动杆菌、保科爱德华菌、类志贺邻单胞菌、腐败希瓦氏菌、嗜麦芽窄食单胞菌、大肠埃希氏菌的检出率则在各批次间存在较大差异。异育银鲫携带的细菌种类及致病性气单胞菌的高携带率对水产养殖与公共卫生造成极大的隐患。研究结果为建立水产养殖细菌性病原(尤其是气单胞菌)监测体系提供参考依据。
An investigation on the diversity of bacterial organisms in hepatopanueas, spleen and kidney of healthy hybridized crussian carp （Carassius auratus gibelio） was carried out in six large-scale breeding farms in Zhejiang province during June--July and September--October in 2013. From a total of 12 bat- ches, seven opportunistic bacterial species capable of affecting humans, animals and fishes were identified. Aerornonas spp. predominated both in June--July and September--October batches and aerA＋act＋ alt act was the major virulence-gene pattern, indicating that the Aeromonas isolates exhibited potential pathogenic inclination. On the contrary, Acinetobacter lwof fii, Edwardsiella hoshinae, Plesio,zonas shigelloides, Shewanella putref aciens, Stenotrophomonas maltophilia and Escherichia coli showed varying isolation rates between June--July and September--October batches. Bacterial species composition and high carry- ing-rate of pathogenic Aeromonas in hybridized prussian carp posed significant risks to aquaculture industry and public health. It is necessary to develop the surveillance system of potential bacterial pathogens, espe- cially Aerornonas spp. , in hybridized crussian carp, and to clarify the pathogenic mechanism to establish comprehensive prevention and control schemes.
出处
《水产科学》
CAS
北大核心
2014年第12期785-789,共5页
Fisheries Science
基金
农业部"浙江省水生动物疫病监控中心"重大建设项目
浙江省海洋与渔业局"水产养殖病害测报"专项资助项目(334006-2010-0006)
关键词
异育银鲫
细菌多样性
气单胞菌
毒力基因型
浙江
hybridized prussian carp
bacterial diversity
Aeromonas
virulence-gene pattern
Zhejiang
