摘要
优选乌梅等三味具有抗胸膜肺炎放线杆菌活性中药的有效物质提取工艺。以浸膏得率、最低抑菌浓度(MIC)和最低杀菌浓度(MBC)为指标,采用正交设计实验,综合考虑溶剂浓度、料液比、提取次数、提取时间、提取温度等影响因素,对三味中药有效物质提取工艺进行优选研究。优选出的最佳提取工艺为:黄连粗粉70%乙醇浓度(8倍量),加热回流提取2次,每次2 h;乌梅粗粉95%乙醇浓度(10倍量),90℃加热回流提取1次,每次8 h;虎杖粗粉60%乙醇浓度(6倍量),90℃加热回流提取3次,每次2 h。最佳提取工艺下,黄连、乌梅和虎杖三种提取物浸膏对胸膜肺炎放线杆菌的MIC值分别为15.60、10.40、15.60 mg/m L,MBC值分别为26.00、18.20、15.60 mg/m L。
This study was designed to obtain the optimized extracting process for the extraction of active substance from 3traditional Chinese medicines with antibacterial activity against Actinobacillus pleuropneumoniae. The orthogonal array design was used with several factors,including solvent concentration,solid-liquid ratio,extraction frequency,extraction duration and extraction temperature. The extraction yield,MIC and MBC were selected as indexes. The results showed that the optimal extraction conditions for Rhizoma coptidis were as follows： 1∶ 8 as the ratio of raw material to liquid,70% as ethanol concentration,and 2 h as extraction duration; the optimal extraction conditions for Fructus mume were as follows∶ 1∶ 10 as the ratio of raw material to liquid,95% as ethanol concentration,90 oC as extraction temperature and 8 h as extraction duration; the optimal extraction conditions for Po Lygonum cuspidatum were as follows∶ 1∶ 6 as the ratio of raw material to liquid,60% as ethanol concentration,90 ℃ as extraction temperature,2 h as extraction duration and reflux extraction for 3 times. Under the optimal extraction conditions,the MIC values of 3 traditional Chinese medicines were15. 60,10. 20 and 15. 60 mg / m L,respectively,and the MBC values were 26. 00,18. 20,15. 60 mg / m L,respectively.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第12期2014-2021,共8页
Natural Product Research and Development
基金
"十二五"农村领域国家科技计划(2011BAD34B03)
四川省青年科技创新研究团队项目(2013TD0015)
水禽疫病控制四川省青年科技创新研究团队项目(2013TD0015)
关键词
乌梅
提取工艺
正交设计
抑菌活性
Fructus mume
extraction process
orthogonal array design
antibacterial activity
