摘要
研究了纳米Fe3 O4协同微生物降解黄褐土中的2，4，6-三氯联苯（PCB30），以PCB30为唯一碳源时降解菌的生长状况，以及微生物接菌量、纳米Fe3 O4投加量、PCB30浓度对黄褐土中PCB30降解的影响。PCBs降解菌经16S rDNA鉴定为假单胞菌Pseudomonas sp．，与草莓假单胞菌Pseudomonas sp．fragi同源性为75％。环境因素对黄褐土中PCB30降解效果有明显影响。微生物接菌量在0～0．8 mL（1×10^9 cfu·mL^－1）、纳米Fe3 O4投加量在0～16．7 g·kg^－1、PCB30浓度在0～10 mg·kg^－1范围内时，PCB30残留率随着微生物接菌量、纳米Fe3 O4投加量以及PCB30浓度的增大而降低。当三者都分别达到各自范围的上限时，微生物单一体系、纳米Fe3 O4单一体系和纳米Fe3 O4／微生物协同体系中PCB30残留率在反应7 d后分别为63．18％、43．27％和26．28％；纳米Fe3 O4／微生物协同体系的降解效果明显优于微生物和纳米Fe3 O4单一体系。
The degradation of 2,4,6-trichlorobiphenyl in alfisol by nanoscale Fe3 O4 combined with microorganism,the growth of cell with PCB30 as the sole carbon source,the effect of cells inoculation amount,nanoscale Fe3 O4 dosage and ini-tial PCB30 concentration were investigated.The PCBs degrader strain was identified as Pseudomonas sp.by 1 6S rDNA gene sequence phylogenetic analysis,and 75% homology with Pseudomonas sp.fragi.The degradation rates of PCB30 increased with the microbial inoculation amount increasing from 0-0.8 mL （1＊1 09 cfu·mL^-1 ）,the nanoscale Fe3 O4 dosage from 0-1 6.7 g·kg^-1 ,and the PCB30 initial concentration from 0-1 0 mg·kg^-1 .When the three parameters reach the upper limit in the regions,the residual rates of PCB30 in the treatments of microorganism,nanoscale Fe3 O4 ,and nanoscale Fe3 O4/microorganism were 63.1 8%,43.27%and 26.28%,respectively.The degradation in the treatment of nanoscale Fe3 O4/mi-croorganism was much better than that in the treatment of nanoscale Fe3 O4 and microorganism.
出处
《世界科技研究与发展》
CSCD
2014年第6期633-637,共5页
World Sci-Tech R&D
基金
高校博士点专项科研基金(20133418110004)
安徽高校省级科学研究项目(KJ2013A108)
安徽省自然科学基金(1208085QD77)资助
