摘要
采用荧光分光光度法,建立了血催化过氧化氢氧化鲁米诺的化学发光体系。考察了缓冲体系、pH值、血液浓度等对鲁米诺化学发光性能的影响。结果表明,当采用56.60mmol·L-1 Na2CO3-NaHCO3缓冲体系与30.0mmol·L-1 H2O2溶液(B液)混合时,在pH=8.98的条件下,血液浓度越高,血催化鲁米诺化学发光性能越好。
Chemiluminescence system of hydrogen peroxide oxidation of luminol catalyzed by blood was establised by fluorescence spectrophotometry.The effect of buffer system,pH value and blood concentration on the chemiluminescence property of luminol were studied.Results showed that,when using 56.60 mmol·L^-1Na2CO3-NaHCO3 as buffer system,mixing with 30.0 mmol·L^-1 H2O2（solution B）,pH value was 8.98,the higher the concentration of blood,the luminol chemiluminescence property catalyzed by blood was better.
出处
《化学与生物工程》
CAS
2014年第12期42-43,66,共3页
Chemistry & Bioengineering
关键词
荧光
鲁米诺
化学发光
性能
fluorescence
luminol
chemiluminescence
property
