摘要
目的探讨芍药苷对沙土鼠脑缺血再灌注(CI/R)损伤的保护作用及其作用机制。方法采用结扎双侧颈总动脉缺血10 min再灌注6 h,建立沙土鼠CI/R模型,随机分为假手术组、模型组、芍药苷3个剂量组(20、10、5 mg·kg-1),每组10只,术前3天开始腹腔注射给药。观察再灌注6 h内神经症状,计算卒中指数;取脑组织匀浆,定磷法测定ATP酶活性,高效液相色谱法测定谷氨酸(Glu)和天冬氨酸(Asp)含量。结果与模型组比较,芍药苷各剂量均能降低CI/R沙土鼠的卒中指数(P<0.05或P<0.01),各剂量均可显著升高脑组织Ca2+-ATP酶活性(P<0.05),高、中剂量组能显著升高Na+-K+-ATP酶活性(P<0.05或P<0.01),各剂量均能降低脑组织Glu含量(P<0.05或P<0.01)。芍药苷对脑组织Mg2+-ATP酶活性和Asp含量则无明显影响。结论芍药苷预处理对CI/R损伤的保护作用与其保护脑细胞膜ATP酶的活性、抑制Glu的释放、降低兴奋性氨基酸毒性等有关。
Objective To investigate the effects ofpaeoniflorin （PAE） on gerbils with cerebral ischemia/reperfusion （CI/R） injury and the mechanisms involved. Methods Gerbil model of CI/R, was prepared by bilateral common carotid occlusion for 10 min followed by 6-hour reperfusion. Then gerbils were divided into 5 groups at random （n=10）, namely sham, model, PAE ihigh, medium and low dose groups （20, 10 and 5 mg·kg^-1, respectively）. PAE was injected intraperitoneally （i.p.） for 3 days before the carotid occlusion. Stroke index was calculated during the reperfusion. The ATPase activities in brain tissue homogenate were examined by a phosphate assay and glutamate （Glu） and aspirate （Asp） contents were determined by HPLC. Results The results showed that PAE significantly improved stroke index, compared to the model group （P 〈0.05 or P 〈0.01）. PAE evidently elevated Ca^2＋-ATPase activities in brain tissue of CUR gerbils （P 〈0.05）. The Na^＋-K^＋-ATPase activities in PAE high and medium dose groups were significantly higher than the model group （P 〈：0.05 or P 〈0.01）. PAE markedly inhibited the Glu contents in brain tissue of CI/R gerbils （P 〈0.05 or P 〈0.01）. However, PAE showed no influence on Mg2^＋-ATPase activities or Asp contents in brain tissue. Conclusion The effectiveness of PAE pretreatment in CI/R injury appears to be associated with the enhancement of ATPase activities and inhibition of excitatory amino acid （EAA） toxicity in brain tissue.
出处
《中国药物警戒》
2014年第12期717-720,共4页
Chinese Journal of Pharmacovigilance
基金
国家自然科学基金(81102870)
关键词
芍药苷
脑缺血再灌注损伤
ATP酶
兴奋性氨基酸
paeoniflorin
cerebral ischemia/reperfusion injury
ATPase
excitatory amino acid（EAA）
