摘要
研究利用液相色谱-串联质谱法（LC-ESI-MS／MS）快速准确测定乳制品中双酚A和壬基酚。通过一系列试验对样品前处理进行了优化研究，样品经乙酸乙酯：环己烷（1：1，体积比）提取净化后，采用HPLC-ESI-MS／MS检测分析，在多反应监测模式（MRM）下，采用内标法定量、方法的检出限为1μg／kg，在1-200μg/kg范围内线性关系良好（相关系数r〉0.99），平均添加回收率为75％-85％，该方法简便、高效、准确，各项技术指标均满足国内外有关双酚A和壬基酚检测要求，可用于乳制品中双酚A和壬基酚的分析测定。
A new method using of ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry for tile analysis of BPA and NP was dew, loped.Sample preparation and HPLC-MS/MS analytical conditions were examined and optimized critically by use of a series of experiments in this study.Samples were extracted by ethyl acetate and cyclohexane,then cleaned up,and uhimately adop｜ed LC-ESI-MS/MS method for qualitative and quantitative analysis.Limits of detection （LOD）was 1 μg/kg, and the method showed good linearity （r〉0.99）between 1μg,/kg and 200μg/kg.The average recoveries were between 75% to 85%.The method is simple,efficient,sensitive and accurate,and it can meet the requirements of determination for BPA and NP.
出处
《现代农业科技》
2015年第1期288-289,291,共3页
Modern Agricultural Science and Technology
关键词
双酚A
壬基酚
乳制品
高效液相色谱-串联质谱
BPA
NP
dairy products
liquid chromatography-electrospray ionization tandem mass spectrometry
