摘要
目的:探讨EBV编码的潜伏膜蛋白2A(LMP2A)与上皮-间质转化(EMT)标志物E-钙粘素(E-Cadherin)和纤维连接蛋白在鼻咽癌(NPC)中的表达及与临床病理学参数的关系。方法:采用免疫组织化学SP法检测LMP2A、E-Cadherin和fibronectin蛋白在32例鼻咽黏膜慢性炎、56例NPC和18例NPC淋巴结转移灶中的表达。结果:1LMP2A在NPC及其淋巴结转移灶中的阳性表达率分别为89.3%(50/56)和77.8%(14/18),均明显高于鼻咽黏膜慢性炎(37.5%,12/32)(均P<0.01);E-Cadherin在NPC及其淋巴结转移灶中的正常表达率分别为33.9%(19/56)和5.6%(1/18),均明显低于鼻咽黏膜慢性炎(90.6%,29/32)(均P<0.01);fibronectin在NPC及其淋巴结转移灶中的阳性表达率分别为83.9%(47/56)和72.2%(13/18),均明显高于鼻咽黏膜慢性炎(28.1%,9/32)(均P<0.01)。2NPC中LMP2A与E-Cadherin正常表达呈负相关(r=-0.387,P<0.01)、与fibronectin表达呈正相关(r=0.421,P<0.01)。3LMP2A、E-Cadherin和fibronectin表达与NPC患者N分期和临床分期密切相关(均P<0.05),与M分期无相关性(P>0.05);LMP2A、E-Cadherin表达与T分期也密切相关(均P<0.01)。结论:LMP2A、fibronectin在NPC中表达升高,而E-Cadherin正常表达则降低;LMP2A可能通过下调E-Cadherin和上调fibronectin表达来介导EMT促进NPC淋巴结转移和恶性进展。
Objective：To investigate the expression of EBV-encoded latent membrane protein 2A （LMP2A） and epithelial-mesenchymal transformation（EMT） associated markers （E-cadherin and fibronectin） in nasopharyn- gem carcinoma （NPC） and its clinical significance. Method;The expression of LMP2A, E-cad-herin and fibronectin proteins in 32 cases of chronic nasopharyngeal inflammat-ion, 56 cases of NPC and 18 eases of NPC lymph node metastasis were examined byimmunohistochemical SP method. Result： @The positive rates of LMP2A in NPC and its lymph node metastasis were significantly higher than those of chronic nasopharyngeal inflammation （89.3 %vs 37.5% and 77.8% vs 37.5%） respectively （both P〈0.01） ; The normal expression rates of E-cadherin in NPC and its lymph node metastasis were significantly lower than those of chronic nasopharyngeal inflammation （33.9 vs 90.6% and 5.6% vs 90.6%） respectively （both P〈0.01）; The positive rates of fibronectin in NPC and its lymph node metastasis were significantly higher than those of chronic nasopharyngeal inflammation（83.9G vs 28.1% and 72.2% vs 28.1%） respectively （both P〈0.01）. @LMP2A expression were negatively correlated with normal expression of E-cadherin （r=- 0. 387, P〈0.01 ）, and were positively correlated with fibronectin （r= 0. 421 ,P〈0. 01）. QLMP2A, E-cadherin and fibronectin expression were significantly correlated with N stage and clinical stage （both P〈0.05）, but the three proteins were not significantly correlated with M stage （both P〉 0.05）. In addition, LMP2A and E-cadherin expression were significantly correlated with T stage （both P〈0.01）. Conclusion： LMP2A and fibronectin expressions were increased in NPC, but normal expression of E-cadherin were decreased. LMP2A may promote lymph node metastasis and malignant progression of NPC by induce EMT through downregulation of E-cadherin and upregulation of fibronectin.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
北大核心
2015年第2期132-136,共5页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
关键词
鼻咽肿瘤
潜伏膜蛋白2A
上皮-间质转化
nasopharyngeal neoplasms
EBV-encoded latent membrane protein 2A
epithelial-mesenchymaltransformation
